基于FCBPSS理论的骨软骨组织工程双相梯度支架的研究

发布时间：2018-03-22 01:06

本文选题：组织工程　切入点：3D生物打印技术　出处：《华东理工大学》2017年硕士论文　论文类型：学位论文

【摘要】：组织工程具有免疫反应小、可以更好的重建原组织形态、结构和功能等优势。应用骨软骨组织工程治疗关节损伤疾病已经成为一种有效途径。组织工程的核心是建立由细胞和支架材料构成的三维空间复合体。三维结构的支架为细胞提供了营养传递和生长代谢的场所,也是形成新的具有形态和功能的组织、器官的基础。然而,目前尚无完备的支架设计理论用于指导支架设计,同时支架体系缺乏纵向层级功能梯度变化,这导致支架无法满足多种环境需求。针对上述支架设计缺陷,本文主要目标为:(1)建立支架设计理论,为支架设计体系的研究提供科学的研究方案;(2)设计一种新型双相梯度三维多孔支架,以满足支架在修复损伤过程中的多种需求。为实现上述目标,本文的主要工作为:(1)将FCBPSS(Function-Context-Behavior-Principle-State-Structure)理论应用于组织工程支架设计领域,针对组织修复系统,提出支架概念设计理论;(2)探索合理支架制备材料组分及配方;(3)设计支架梯度结构,并验证其结构强度;(4)采用3D生物打印技术制备双相梯度支架,进行支架性能检测。通过以上工作,本文得到的主要结论为:(1)FCBPSS理论可科学有效的指导骨软骨修复系统中的支架设计,为支架设计制备提供科学的理论支撑;(2)由明胶/透明质酸(GelMA/HA)所组成的水凝胶体系作为支架软骨相,聚乳酸-羟基乙酸/聚乙二醇(PLGA/PEG)复合体系作为支架骨相,可利于3D打印支架制备,同时适宜细胞生长增殖分化;(3)本文中所建立梯度结构的支架设计方案满足人体力学环境强度需求。本文的主要贡献在于:(1)在支架设计理论方面,将FCBPSS理论作为支架设计的指导理论,为支架设计提供科学的依据;(2)设计了一种新型双相三维多孔梯度支架,并验证支架设计科学性,为组织工程应用的发展做了进一步科学探索和贡献。
[Abstract]:Tissue engineering has a small immune response, which can better reconstruct the original tissue morphology. Structural and functional advantages. The application of osteochondral tissue engineering in the treatment of joint injury has become an effective approach. The core of tissue engineering is the establishment of three-dimensional space complex composed of cells and scaffolds. The scaffold provides a place for cells to transmit nutrients and grow and metabolize, It is also the basis for the formation of new tissues and organs with shape and function. However, there is no complete scaffold design theory to guide the scaffold design, and the scaffold system lacks the vertical level functional gradient change. In view of the defects of the above scaffold design, the main goal of this paper is to establish the scaffold design theory. To provide a scientific research scheme for the study of scaffold design system, a new type of biphasic gradient three-dimensional porous scaffold is designed to meet the various needs of the scaffold in the process of repairing damage. The main work of this paper is to apply the FCBPSSS-Function-Context-Behavior-Principle-State-Structure Theory to the field of scaffold design in tissue engineering. For the tissue repair system, a conceptual scaffold design theory is proposed. The biphasic gradient scaffold was prepared by using 3D biometric printing technology, and the performance of the scaffold was tested. The main conclusion of this paper is that the FCBPSS theory can be used to scientifically and effectively guide the design of scaffolds in bone cartilage repair system. The hydrogel system composed of gelatin / hyaluronic acid Gelma / HA was used as the scaffold cartilage phase, and the poly (lactic acid-glycolic acid / poly (ethylene glycol) / PLGA / PEG) composite system was used as the scaffold bone phase. It can be used for 3D printing scaffold preparation, and is suitable for cell growth, proliferation and differentiation. The scaffold designed with gradient structure in this paper can meet the needs of human mechanical environment strength. The main contribution of this paper is in the aspect of scaffold design theory. Taking the FCBPSS theory as the guiding theory of support design and providing a scientific basis for the design of scaffolds, a new type of biphasic three-dimensional porous gradient scaffold is designed, and the scientific design of the scaffold is verified. Further scientific exploration and contribution have been made for the development of tissue engineering application.
【学位授予单位】：华东理工大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R318.08

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