壳聚糖藻酸盐支架与脂肪间充质干细胞复合构建的组织工程脊髓修复急性脊髓损伤

发布时间：2018-03-22 15:52

本文选题：壳聚糖　切入点：藻酸盐　出处：《中国组织工程研究》2017年26期 　论文类型：期刊论文

【摘要】：背景:组织工程化脊髓为脊髓损伤的修复带来了新的治疗思路。目的:探讨壳聚糖藻酸盐支架与脂肪间充质干细胞复合的组织工程脊髓修复大鼠急性脊髓损伤的效果。方法:取48只SD大鼠,建立脊髓半横断模型,随机分4组干预,模型组直接缝合硬膜,细胞组在脊髓断端周边移植脂肪间充质干细胞,支架组在脊髓损伤区植入壳聚糖藻酸盐支架,实验组在脊髓损伤区植入组织工程脊髓(壳聚糖藻酸盐支架与CM-Dil标记脂肪间充质干细胞复合物)。移植后1,3,7周,评估大鼠后肢运动功能;移植后7周取脊髓标本,进行免疫荧光染色和苏木精-伊红染色。结果与结论:(1)后肢运动功能:移植后3,7周,实验组后肢运动功能强于模型组、细胞组及支架组(P0.05),细胞组、支架组后肢运动功能强于模型组(P0.05);(2)免疫荧光染色:模型组无神经丝蛋白200、神经元特异性烯醇化酶、胶原纤维酸性蛋白阳性细胞;支架组可见神经元特异性烯醇化酶阳性细胞、胶原纤维酸性蛋白阳性细胞及少量神经丝蛋白200阳性细胞;细胞组未见神经丝蛋白200、神经元特异性烯醇化酶阳性细胞,可见大量的胶原纤维酸性蛋白阳性细胞;实验组见大量神经元特异性烯醇化酶阳性细胞,少量CM-Dil标记的脂肪间充质干细胞、较多的胶原纤维酸性蛋白阳性细胞及神经丝蛋白200阳性细胞;(3)苏木精-伊红染色:模型组有大量成纤维细胞和炎性细胞浸润,有较大的脊髓空洞;支架组无脊髓空洞,脊髓和支架材料之间连接紧密;细胞组有脊髓空洞;实验组无脊髓空洞,脊髓损伤区有各种形态细胞形成;(4)结果表明:壳聚糖藻酸盐支架与脂肪间充质干细胞复合的组织工程脊髓,可促进急性脊髓损伤的修复。
[Abstract]:Background: tissue engineered spinal cord provides a new therapeutic approach for the repair of spinal cord injury. Objective: to investigate the effect of chitosan alginate scaffold combined with adipose mesenchymal stem cells on the repair of acute spinal cord injury in rats. Methods: 48 SD rats were selected. The model of spinal cord hemitransection was established and randomly divided into 4 groups. The model group directly sutured the dura mater, the cell group transplanted adipose mesenchymal stem cells around the broken end of spinal cord, and the scaffold group implanted chitosan alginate scaffold in the injured area of spinal cord. The experimental group implanted tissue engineered spinal cord (chitosan alginate scaffold and CM-Dil labeled adipose mesenchymal stem cell complex) in the spinal cord injury area. Results and conclusion: the motor function of hind limbs in the experimental group was stronger than that in the model group, the cell group and the scaffold group (P0.05, cell group) at 3 ~ 7 weeks after transplantation, the hind limb motor function of the experimental group was stronger than that of the model group, and that of the cell group and the scaffold group was higher than that of the control group. The motor function of the hind limbs in the stent group was stronger than that in the model group (P 0.05) immunofluorescence staining: there were no neurofilament protein 200, neuron-specific enolase, collagen fibrillary acidic protein positive cells in the model group, and neuron-specific enolase positive cells were found in the stent group. Collagen fibrillary acidic protein positive cells and a few neurofilament protein 200 positive cells were not found in the cell group, and a large number of collagen fibrillary acidic protein positive cells were found in the cell group, no neurofilament protein 200 and neuron-specific enolase positive cells were found in the cell group. A large number of neuron-specific enolase positive cells and a few CM-Dil labeled adipose mesenchymal stem cells were found in the experimental group. More collagen fibrillary acidic protein positive cells and neurofilament protein 200 positive cells 3) hematoxylin-eosin staining: model group has a large number of fibroblasts and inflammatory cells infiltration, there is a large syringomyelia, stent group has no syringomyelia, The connection between the spinal cord and the scaffold material was tight; the cell group had syringomyelia; the experimental group had no syringomyelia. The results showed that the composite of chitosan alginate scaffold and adipose mesenchymal stem cells could promote the repair of acute spinal cord injury.
【作者单位】：西南医科大学附属中医医院骨科;川北医学院附属医院骨科;
【基金】：四川省教育厅项目(16ZB0229)~~
【分类号】：R318.08;R651.2

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