阵列式化学传感器点样仪的研究设计

发布时间：2018-03-22 20:38

本文选题：阵列式化学传感器　切入点：点样仪　出处：《重庆大学》2015年硕士论文　论文类型：学位论文

【摘要】：随着化学传感器技术的发展,对于简单、便捷、快速和应用广泛的检测分析方法的迫切需求,阵列式化学传感器应运而生,并且在科学研究和实际生活中得到了广泛应用,尤其体现在分析检测复杂化合物方面。由于阵列式化学传感器具有灵敏度高、响应速度快和可集成度高等优点,被广泛应用食品、糖类、有毒气体和疾病等领域的检测。大量研究实验表明卟啉及衍生物作为传感元件,具有很高的灵敏度和化学稳定性,在对目标物的特异性识别与鉴定方面体现出特殊功能,因此,如何构建可靠的可视化卟啉化学传感阵列,对多学科交叉领域的研究具有很重要的意义。生成像如可视化卟啉化学传感阵列的试剂多具有腐蚀性、易挥发性、混合性的特点。阵列中每个样点的试剂成分不同,为了保持每个样点直径相近,制备过程中存在不同试剂扩散速度不同的问题。当前市面上及专利报道中没有专门制备这种特殊阵列的点样装置。在功能方面唯一比较接近的是生物芯片点样仪,但其点样对象主要是核酸、蛋白质、抗体和组织,因此生物芯片点样仪不可直接制备这种阵列式化学传感器。有鉴于此,本课题旨在提供一套可以自动制备阵列式化学传感器的点样仪。该仪器可以自动吸取高腐蚀性试剂,自动完成点样,并且所制化学传感器满足试验要求。本文的主要工作有一下几个方面:一、根据现有接触式生物芯片点样仪的工作原理,设计阵列式化学传感器点样仪的整体结构。该结构主要满足以下几个方面的要求:1、实现X、Y轴两个方向的自由运动;2、点样头可以加装36根半径不同的点样针;3、可以实现36根点样针同时加样;4、可以连续制备多张化学传感器;5、试剂槽可以盛装高腐蚀性化学试剂。二、根据点样仪的整体结构及点样仪应具有的功能,设计了以PIC16F877A单片机为控制核心的硬件系统,包括矩阵键盘模块、液晶显示模块、电源模块、定位检测模块、步进电机驱动电路模块。根据各模块的功能,在MPLAB中完成整个控制系统的软件编写。三、不同试剂在相同基底上的扩散速度不同,因此,在点样过程中存在不同液体在相同时间内无法扩散相同距离的问题。本文根据毛细管流体模型、Washburn方程、Laplace方程等,建立了液体在基底上的扩散模型,保证不同液体在相同时间内扩散相同的距离。四、测试系统各个模块,并将各功能模块组装,对样机进行调试。五、通过对比点样仪制备的传感器、手工制备的传感器分别与VOCs反应后两种阵列差谱图中各敏感点的欧式距离和样本相对标准偏差,验证点样仪制备传感器比手工制备传感器更稳定,与VOCs响应更强烈。
[Abstract]:With the development of chemical sensor technology and the urgent need for simple, convenient, fast and widely used detection and analysis methods, the array chemical sensor has emerged as the times require, and has been widely used in scientific research and practical life. Especially in the analysis and detection of complex compounds. Because of its high sensitivity, high response speed and high integration, the array chemical sensor has been widely used in food, sugar, A large number of experiments show that porphyrin and its derivatives as sensing elements have high sensitivity and chemical stability, and exhibit special functions in the specific identification and identification of the target. How to construct a reliable visual porphyrin chemical sensing array is of great significance to the research of multi-disciplinary cross field. The reagents like visual porphyrin chemical sensing array are corrosive and volatile. Hybrid characteristics. The composition of each sample in the array is different, in order to keep the diameter of each sample close, There is a problem of different reagents diffusing at different speeds in the preparation process. At present, there is no spot device specially prepared for this special array in the market and in patent reports. The only thing that is relatively close in function is the biochip sampler. But its sample is mainly nucleic acid, protein, antibody and tissue, so the biochip spotting instrument can not directly produce this kind of array chemical sensor. The purpose of this paper is to provide a set of sampling instruments that can automatically prepare array chemical sensors. The instrument can automatically absorb highly corrosive reagents and finish the sampling automatically. The main work of this paper is as follows: first, according to the working principle of the existing contact biochip sampler, The overall structure of the array chemical sensor spotting instrument is designed. The structure mainly meets the following requirements: 1: 1, realizes the free movement of XY axis in both directions, and the spot head can be fitted with 36 sampling needles with different radii. It is realized that 36 sample needles can be added to sample 4 simultaneously, and multiple chemical sensors can be prepared continuously. The reagent tank can be filled with highly corrosive chemical reagents. According to the whole structure of the sampling instrument and the function of the sampling instrument, a hardware system based on PIC16F877A microcontroller is designed, which includes matrix keyboard module, liquid crystal display module, power supply module, location detection module, etc. Stepper motor drive circuit module. According to the function of each module, complete the whole control system software in MPLAB. Third, different reagents on the same substrate diffusion speed is different, therefore, In this paper, the diffusion model of liquid on the substrate is established according to the Washburn equation and Laplace equation of capillary fluid. Ensure that different liquids diffused at the same distance within the same time. Fourthly, each module of the test system is assembled, and the prototype is debugged. The Euclidean distance and the sample relative standard deviation of each sensitive point in the two kinds of array differential spectrum diagram after the reaction of the hand-made sensor with VOCs, respectively. It is verified that the sensor prepared by the spot detector is more stable than that of the sensor prepared by hand, and the response of the sensor is stronger than that of the VOCs sensor.
【学位授予单位】：重庆大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：TP212.2;R318

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