多聚甲醛固定对大鼠椎骨黏弹性影响的实验研究

发布时间：2018-03-23 04:19

  本文选题：黏弹性　切入点：应力松弛　出处：《医用生物力学》2017年04期 　论文类型：期刊论文

【摘要】：目的观察多聚甲醛固定对大鼠椎骨黏弹性力学性能的影响,从生物力学角度寻找松质骨样品的最佳保存方式。方法选择8周龄健康雌性SD大鼠20只,手术分离获得完整的腰椎L4和L5共40只,随机平均分为固定组和对照组,固定组置于4%多聚甲醛中固定72 h,对照组置于5 m L EP管内-20℃冷冻保存。两组随机各取10只分别行应力松弛和蠕变实验,7 200 s后收集样品并通过显微CT分析其微观结构变化。结果固定组松弛蠕变曲线相比对照组更加平滑,达到稳态的时间较短,且500 s和7 200 s的松弛蠕变总量明显降低(P0.01)。显微CT结果显示松弛蠕变实验会引起骨小梁断裂,且固定组比对照组骨小梁破坏更加严重。结论多聚甲醛固定法显著降低大鼠椎骨的黏弹性,在力学负荷下更易造成其微观结构破坏,不利于松质骨保存。
[Abstract]:Objective to observe the effect of paraformaldehyde fixation on the viscoelastic properties of vertebrae in rats and to find out the best preservation method of cancellous bone samples from the biomechanical point of view. Methods 20 healthy female SD rats aged 8 weeks were selected. A total of 40 lumbar vertebrae L4 and L5 were isolated and randomly divided into two groups: the fixed group and the control group. The fixation group was fixed in 4% paraformaldehyde for 72 hours, and the control group was frozen in 5 mL EP tube at -20 鈩，

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