含壳聚糖微球的壳聚糖—聚己内酯支架用于TGF-β1因子的控制释放研究

发布时间：2018-03-24 00:32

本文选题：壳聚糖-聚己内酯　切入点：壳聚糖微球　出处：《华中科技大学》2013年硕士论文

【摘要】：采用基团保护方法合成了壳聚糖-聚己内酯共聚物。在优化条件下，聚己内酯侧链的接枝率可达到约70%。对壳聚糖-聚己内酯进行了表征。合成产物的化学结构和物理性质通过红外光谱，核磁共振光谱进行定性。以京尼平作为交联剂，通过乳化交联技术制备了载TGF-β1因子的壳聚糖微球。利用扫描电子显微镜观察了微球的形态和尺寸，通过图像分析系统测定了微球的粒径分布和平均直径，筛选出粒径低于45μm微球用于支架/微球复合物制备。通过调节固化过程中交联剂的用量，可以使微球的包封率达到80%以上。将筛选出的载TGF-β1微球复合到壳聚糖-聚己内酯共聚物疏松多孔支架中，研究发现通过改变CH-PCLS的组成比例和支架的结构参数等可有效控制支架的机械强度和因子的释放。聚己内酯的含量控制在42wt.%左右可以得到较好的效果。通过体外模拟释放实验得出高于80%孔隙率的支架材料、初始载药量为3ng(TGF-β1)/mg(支架重量)的支架可以实现TGF-β1因子的控制释放超过四周，，且不具有明显的突释性。经膨胀性质和力学性质测量得出这种类型的微球/壳聚糖-聚己内酯支架复合体在湿态下具有较好的好的压缩力学性能。
[Abstract]:Chitosan-polycaprolactone copolymers were synthesized by group protection method. The graft rate of the side chain of polycaprolactone was about 70%. The chemical structure and physical properties of the synthesized products were characterized by IR and NMR spectra. Chitosan microspheres loaded with TGF- 尾 1 were prepared by emulsification and crosslinking with genipine as crosslinking agent. The morphology and size of the microspheres were observed by scanning electron microscope (SEM). The particle size distribution and average diameter of the microspheres were measured by image analysis system. The microspheres with diameter less than 45 渭 m were selected for the preparation of scaffold / microsphere composites. The amount of crosslinking agent was adjusted during the curing process. The encapsulation efficiency of the microspheres can reach more than 80%. The selected TGF- 尾 1 microspheres were mixed into the porous scaffolds of chitosan / polycaprolactone copolymers. It is found that the mechanical strength and factor release of the scaffold can be effectively controlled by changing the composition ratio of CH-PCLS and the structural parameters of the scaffold, and the content of polycaprolactone can be controlled at about 42 wt.%. Pseudo-release experiments show that scaffold materials with a porosity of more than 80%, A stent with an initial dose of 3ng-1 TGF- 尾 1 / mg (stent weight) can control the release of TGF- 尾 1 factor for more than four weeks. It was found that the microspheres / chitosan-polycaprolactone scaffolds had good compressive mechanical properties under wet conditions.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R318.08

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