基于离子交换响应型纳米银复合物的制备及抗菌性能研究

发布时间：2018-03-26 07:26

本文选题：纳米银　切入点：海藻酸凝胶　出处：《太原理工大学》2014年硕士论文

【摘要】：纳米银抗菌凝胶是由纳米银和高分子材料构成的复合物。近些年来,纳米银抗菌凝胶作为一种有效的抗菌材料被广泛地应用于临床。最近的研究结果显示,过量的纳米银容易导致细胞毒性。因此,如何有效地控制复合物中纳米银的释放水平,以期实现抗菌效果,并避免细胞毒性,成为当前纳米银抗菌凝胶所要解决的一个关键问题。本研究采用海藻酸钠作为载体,通过原位还原反应、凝胶化、梯度脱水、真空干燥等过程,形成海藻酸凝胶-纳米银复合物(Alg-AgNPs),海藻酸的作用有二。一是作为合成并固定纳米银颗粒的载体,从而避免了纳米银颗粒聚集而失去其纳米效应。二是海藻酸凝胶作为环境敏感性材料,实现纳米银的控制释放。复合物的杀菌性能及细胞毒性决定于纳米银颗粒的尺寸及浓度,结果显示,在该体系中纳米银颗粒的平均尺寸为2-6m,均匀地分散在凝胶中,当该复合物与模拟体液接触后,体液中的Na+与复合物中的Ca2+发生离子交换,释放出纳米银颗粒,纳米银颗粒的释放水平取决于复合物中AgNPs的装载量及体液的量。当介质中该纳米银颗粒的浓度达到1.1ppm时,能实现有效的杀菌性能(4小时内杀灭107大肠杆菌和金黄色葡萄球菌),并且杀菌效率与纳米银颗粒的浓度呈正相关。而随着纳米颗粒浓度的增大,逐步会导致细胞坏死。因此,控制纳米银颗粒浓度不高于1.1ppm可实现抗菌并避免细胞毒性。该复合物通过离子交换响应实现对纳米银颗粒释放的智能控释,实现了抗菌性能,并避免了细胞毒性,为纳米银抗菌材料的研究提供了一个新思路。
[Abstract]:Nano-silver antibacterial gel is a complex composed of nano-silver and polymer materials. In recent years, nano-silver antibacterial gel has been widely used in clinic as an effective antibacterial material. Excessive amounts of silver nanoparticles can easily lead to cytotoxicity. Therefore, how to effectively control the release level of silver nanoparticles in the complex in order to achieve antibacterial effect and avoid cytotoxicity, It has become a key problem to be solved by nano-silver antimicrobial gel. In this study, sodium alginate was used as carrier, through in situ reduction reaction, gelation, gradient dehydration, vacuum drying and so on. To form alginate gel-nano-silver complex Alg-AgNPsN, alginic acid acts as a carrier for the synthesis and immobilization of silver nanoparticles. Therefore, the aggregation of silver nanoparticles and the loss of their nanometer effects are avoided. Second, alginate gel is used as an environmentally sensitive material to realize the controlled release of silver nanoparticles. The bactericidal properties and cytotoxicity of the complexes are determined by the size and concentration of silver nanoparticles. The results showed that the average size of silver nanoparticles in the system was 2-6 m, which was uniformly dispersed in the gel. After contact with the simulated body fluid, the Na in the body fluid and the Ca2 in the complex produced ion exchange, resulting in the release of silver nanoparticles. The release level of silver nanoparticles depends on the amount of AgNPs in the composite and the amount of body fluid. When the concentration of the nanoparticles in the medium reaches 1.1ppm, It can achieve effective bactericidal performance and kill 107 Escherichia coli and Staphylococcus aureus within 4 hours, and the bactericidal efficiency is positively correlated with the concentration of silver nanoparticles. Controlling the concentration of silver nanoparticles below the concentration of 1.1ppm can achieve antimicrobial activity and avoid cytotoxicity. The complex can realize intelligent controlled release of silver nanoparticles by ion exchange response, achieve antibacterial properties, and avoid cytotoxicity. It provides a new idea for the research of nano-silver antibacterial materials.
【学位授予单位】：太原理工大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R318.08

【参考文献】