小RNA含量的检测和葡萄糖酸调控纳米金结构的合成

发布时间：2018-03-26 18:18

本文选题：MicroRNAs　切入点：癌症　出处：《南京农业大学》2013年硕士论文

【摘要】：MicroRNA是最初在对秀丽线虫的研究中发现的,现阶段,microRNA被广泛发现已存在于细菌、真菌及多种生物体内。通常来说,MicroRNA是21-22核苷酸构成的内源性的不编码任何蛋白的单链RNA,通过与靶标mRNA3UTR,5UTR和开放阅读框结合,来调节mRNA的翻译或降解,以达到调控基因表达的作用,从而参与细胞的分化、增殖、代谢和自噬过程。人体中含有至少500种miRNAs,并以此来调控正常的细胞周期。基于miRNAs在生物体内的多种调控,建立适合的方法进行miRNAs勺检测方法对于细菌、真菌以及一些疾病的研究具有重要意义。本文中利用滚环扩增和切刻酶信号放大的方法,对食管鳞癌组织及其旁组织中几种miRNAs含量进行了检测,结果发现,不同样品中含量的表达具有差异性,总体来说,癌组织中这几种miRNAs的含量高于旁组织,证明了这几种miRNA的不正常表达与食管鳞癌的产生存在某种关系。 AuNPs是一种多用途的纳米材料,其具有良好的生物相容性,能与多种生物大分子结合并且不影响其生物活性。在许多的研究中,纳米金被用于生物探针的制备,并进行核酸及细菌检测的研究。但是纳米金在制备过程中往往采用具有毒性的表面活性剂,制约了其在细菌及细胞实验中的应用。研究表明,葡萄糖酸可作为钝化剂吸附在金的特定位面,基于这个原因,我们利用葡萄糖酸作为表面钝化剂和还原剂,调节浓度、温度、pH、O2等条件来研究其对合成纳米材料的影响,希望制备出低毒性的纳米金颗粒以期用于micRNAs的检测。但是,我们发现,虽然在改变了一些参数后可以合成一些三角形或六边形的片层结构,但是产率一直很低,并且生成产物很不均一,证明了葡萄糖酸虽然可以特异的吸附某些晶面,但是这种保护能力并不是很高,因此不能完全控制产物的形貌。
[Abstract]:MicroRNA was first found in the study of nematodes, and microRNAs are now widely found in bacteria. Generally speaking, microRNAs are endogenous single-stranded RNAs consisting of 21-22 nucleotides that do not encode any protein, regulating the translation or degradation of mRNA by binding to the target mRNA3UTR5UTR and open reading frame, in order to regulate gene expression. In order to participate in cell differentiation, proliferation, metabolism and autophagy. The human body contains at least 500 kinds of miRNAss, which is used to regulate the normal cell cycle. It is of great significance to establish a suitable method for the detection of miRNAs spoons for the study of bacteria, fungi and some diseases. The contents of miRNAs in esophageal squamous cell carcinoma and its adjacent tissues were detected. The results showed that there were differences in the expression of miRNAs in different samples. In general, the contents of these miRNAs in cancer tissues were higher than those in adjacent tissues. It is proved that the abnormal expression of miRNA is related to the production of esophageal squamous cell carcinoma. AuNPs is a multi-purpose nano-material with good biocompatibility, can bind to many biological macromolecules without affecting its biological activity. In many studies, nano-gold has been used in the preparation of biological probes. Nucleic acid and bacterial detection were also studied. However, in the preparation of gold nanoparticles, toxic surfactants were often used, which restricted their application in bacteria and cell experiments. Gluconic acid can be used as passivator to adsorb on the specific surface of gold. For this reason, we use gluconic acid as surface passivator and reductant, adjust the concentration, temperature and pH of oxygen to study the effect of gluconic acid on the synthesis of nanomaterials. We hope to prepare low toxicity gold nanoparticles for the detection of micRNAs. However, we found that although some parameters can be changed to synthesize some triangular or hexagonal lamellar structure, the yield has been very low. And the product is very heterogeneous, which proves that gluconic acid can adsorb some crystal planes, but the protective ability is not very high, so it can not completely control the morphology of the product.
【学位授予单位】：南京农业大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R318.08

【共引文献】