钛表面层层自组装肝素和白蛋白及其力学变形行为研究

发布时间：2018-03-26 18:44

本文选题：肝素　切入点：白蛋白　出处：《西南交通大学》2012年硕士论文

【摘要】：本论文通过层层静电自组装法在超薄的高纯钛表面交替组装肝素和白蛋白分子层。高纯钛通过碱活化后表面呈负电性,多聚赖氨酸在PBS溶液中呈正电性,通过静电作用可将多聚赖氨酸吸附于碱活化的钛表面,然后将其交替浸泡于带负电的肝素溶液和带正电的白蛋白溶液中形成多层自组装膜。最后在不同弯曲半径下使之受力弯曲,通过傅里叶红外漫反射(FTIR)检测表面基团的变化,通过接触角检测对组装过程中表面亲水性以及表面能的变化进行表征,通过激光共聚焦显微镜和扫描电子显微镜观察材料表面形貌的变化,以了解外力作用对组装生物分子后的材料表面理化性能和血液相容性影响。 (1)傅里叶红外漫反射(FTIR)结果显示肝素和白蛋白成功的组装到钛表面。水接触角结果进一步表明各步处理成功。随着组装层数的增加,水接触角的值呈上升的趋势。 (2)激光共聚焦显微镜结果表明,钛表面组装的生物分子层在基底材料弯曲变形的过程中,有裂纹出现。 (3)扫描电镜结果表明,在弯曲直径为Φ5mm,即材料延伸率为1.6%的时候,出现明显裂纹,且随着膜厚的增加,裂纹数量增多,且有扩展的趋势。 (4)血小板黏附实验表明,弯曲变形后,表面血小板黏附数量有一定增加,其中弯曲直径为φ15mm和φ10mm时,血小板数量最多。变形后,在不同弯曲直径条件下,血小板的聚集程度增加。无论变形前后,对于组装7层、12层、17层的样品,17层血小板黏附数量最少。综上所述,构建了生物修饰层的钛表面在受到外力作用而弯曲的时候,其表面性质发生改变。
[Abstract]:In this paper, the layer by layer electrostatic self-assembly method was used to assemble heparin and albumin molecular layers alternately on the surface of ultra-thin high-purity titanium. The surface of high-purity titanium was negatively charged after alkali activation, and poly-lysine was positively electrically present in PBS solution. Polysine can be adsorbed on the surface of alkaline activated titanium by electrostatic action. Then they were immersed alternately in a negatively charged heparin solution and a positively charged albumin solution to form a multilayer self-assembled film. Finally, the surface groups were measured by Fourier transform infrared diffuse reflectance (FTIR). The changes of surface hydrophilicity and surface energy during assembly were characterized by contact angle measurement, and the changes of surface morphology were observed by laser confocal microscope and scanning electron microscope. In order to understand the effect of external force on the surface physical and chemical properties and blood compatibility of the biomolecules assembled. The FTIR results showed that heparin and albumin were successfully assembled on the titanium surface. The water contact angle showed that the water contact angle increased with the increase of the number of the assembled layers. (2) the results of laser confocal microscopy show that there are cracks in the biomolecular layer of titanium surface during the bending deformation of the substrate material. The SEM results show that obvious cracks appear when the bending diameter is 桅 5mm, that is, the elongation of the material is 1.6%, and with the increase of film thickness, the number of cracks increases and tends to propagate. 4) Platelet adhesion test showed that the number of platelet adhesion on the surface increased after bending deformation, especially when the bending diameter was 蠁 15mm and 蠁 10mm, the number of platelets was the most when the bending diameter was 蠁 15mm and 蠁 10mm. The platelet aggregation was increased. No matter before and after deformation, the number of platelet adhesion in 17 layers was the least for the sample of 7 layers and 12 layers and 17 layers. In conclusion, the surface properties of the modified titanium surface changed when it was bent by external force.
【学位授予单位】：西南交通大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.08

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