以牛心包为来源的天然衍生引导骨组织再生膜制备方法的实验研究

发布时间：2018-03-27 05:15

本文选题：天然衍生牛心包脱细胞处理交联处理　切入点：GBR　出处：《口腔医学研究》2016年05期

【摘要】：目的:探索天然牛心包脱细胞和交联处理的理想方法。方法:用胰酶去垢剂法(I)、胰酶核酸酶法(II)、胰酶去垢剂+核酸酶联合法(III)、冻融去污剂法(IV)、冻融核酸酶法(V)、冻融去污剂+核酸酶联合法(VI)进行脱细胞和交联处理,采用光镜和SEM、细胞毒性实验、机械性能测定、细胞趋化性实验评估处理效果,筛选出理想的脱细胞和交联方法。结果:冻融去污剂+核酸酶联合法是理想的脱细胞方法。京尼平交联法是理想的交联方法。结论:冻融去污剂+核酸酶联合法制备的脱细胞牛心包再采用京尼平交联法可制备出理想的GBR膜材料。
[Abstract]:Objective: to explore an ideal method for natural bovine pericardial decellularization and cross-linking. Methods: using trypsin scale removing agent method, trypsin nuclease enzyme method, trypsin descaling agent nuclease enzyme method, trypsin descaling agent nuclease enzyme method, freeze-thawing detergent method, freeze-thawing agent method, freeze-thawing enzyme method, freeze-thawing nucleic acid enzyme method, freeze-thawing enzyme method. The decellularization and crosslinking were carried out by melt-leachate nuclease linked assay (Vi). Light microscope and SEM, cytotoxicity test, mechanical property test and chemotaxis test were used to evaluate the effect of treatment. Results: freezing and thawing agent nucleic acid enzyme-linked method is an ideal acellular method, genipine cross-linking method is an ideal cross-linking method. Conclusion: freeze-thaw detergent nucleic acid enzyme-linked method is an ideal cross-linking method. The acellular bovine pericardium prepared by genipin crosslinking method can be used to prepare ideal GBR membrane material.
【作者单位】：长沙市口腔医院正畸科;中南大学湘雅二医院检验科;长沙市口腔医院牙周科;中南大学湘雅二医院口腔科;四川大学华西口腔医院;
【基金】：长沙市科技局项目(编号:0803160-32)
【分类号】：R318.08

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