二氧化钛纳米管生物透析膜的生物相容性

发布时间：2018-03-28 12:24

本文选题：肾透析　切入点：溶血　出处：《中国组织工程研究》2017年26期

【摘要】：背景:传统的透析膜多由有机聚合物材料合成,这些材料虽然能满足治疗需要,但材料孔径尺寸范围较大,微管不均匀,容易造成蛋白质在透析膜上淤塞,难以达到预期的治疗效果。目的:分析二氧化钛纳米管生物透析膜的血液相容性。方法:根据阳极氧化法制备高强度的二氧化钛纳米管薄膜,利用HF气体对纳米管底部进行腐蚀,获得两端通透的二氧化钛纳米管阵列薄膜,将LLC-PK1细胞和ECV304细胞分别接种于二氧化钛纳米管阵列薄膜上,获得两种二氧化钛纳米管生物透析膜。(1)溶血实验:将两种二氧化钛纳米管生物透析膜及聚氨酯材料分别浸泡于生理盐水与蒸馏水中,检测溶血率;(2)动态凝血时间实验:将涂过兔血的两种二氧化钛纳米管生物透析膜及聚氨酯材料分别与氯化钙混合,计算动态凝血时间;(3)血小板黏附实验:将富血小板血浆分别滴于两种二氧化钛纳米管生物透析膜及聚氨酯材料上,计算血小板黏附率。结果与结论:(1)溶血实验:接种LLC-PK1细胞、ECV304细胞通透二氧化钛纳米管薄膜的溶血率均低于聚氨酯材料(0.30%,0.34%,0.56%,P0.05);(2)动态凝血时间实验:接种LLC-PK1细胞、ECV304细胞通透二氧化钛纳米管薄膜20,40,70 min的动态凝血时间显著长于聚氨酯材料(P0.05);(3)血小板黏附实验:接种LLC-PK1细胞、ECV304细胞通透二氧化钛纳米管薄膜的与聚氨酯材料相比差异无显著性意义(P0.05);(4)结果表明:二氧化钛纳米管生物透析膜具有良好的血液相容性。
[Abstract]:Background: traditional dialysis membranes are mostly synthesized from organic polymer materials. Although these materials can meet the needs of treatment, the pore size of the materials is large, the microtubules are uneven, and proteins are easily silted up on the dialysis membranes. Objective: to analyze the blood compatibility of titanium dioxide nanotube biodialysis membrane. Methods: high strength titanium dioxide nanotube film was prepared by anodic oxidation method. TIO _ 2 nanotube array films were obtained by HF gas corrosion on the bottom of nanotubes. LLC-PK1 cells and ECV304 cells were inoculated on TIO _ 2 nanotube array films, respectively. Two kinds of titanium dioxide nanotube biodialysis membranes were obtained. The hemolysis experiments were carried out. Two kinds of titanium dioxide nanotube biodialysis membranes and polyurethane materials were immersed in saline and distilled water respectively. Dynamic Coagulation time experiment: two kinds of titanium dioxide nanotube biodialysis membranes and polyurethane materials coated with rabbit blood were mixed with calcium chloride respectively. Platelet adhesion experiment: platelet-rich plasma was dripped on two kinds of titanium dioxide nanotube biodialysis membrane and polyurethane material respectively. Results and conclusion\\\. The dynamic coagulation time of the permeable titanium dioxide nanotube film 2040 / 70 min was significantly longer than that of the polyurethane material (P0.05 / 3) platelet-adhesion test: there was no significant difference in the penetration of TiO2 nanotube film between the LLC-PK1 cells and the polyurethane material after inoculation of LLC-PK1 cells with ECV304 cells. The results showed that the titanium dioxide nanotube biodialysis membrane had good blood compatibility.
【作者单位】：新乡医学院;
【基金】：河南省科技发展攻关项目(2014HNA0032)~~
【分类号】：R318.08

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