超支化聚合物新材料富集糖肽方法的研究

发布时间：2018-03-28 14:55

  本文选题：HHMs　切入点：糖肽　出处：《中国生物工程杂志》2014年08期

【摘要】：目的:将自合成的超支化聚合物固定到氨基硅球上形成一种新型的糖肽富集材料,用于糖肽的高效分离与富集。方法:阴离子开环聚合法合成的超支化聚缩水甘油,经高碘酸氧化成为超支化聚甘油醛(hyperbranched polyglycerol-aldehydes,HPG-ALD)后固定于氨基硅球上,再用己二酸二酰肼修饰得到的酰肼材料应用于糖肽富集。结果:经扫描电镜(Scanning Electron Microscope,SEM)、热重分析(thermogravimetric analysis,TGA)等手段证明材料被成功合成。同时,使用制备成功的新材料来富集标准糖蛋白和糖肽都获得了良好的富集效果。结论:实验证明超支化缩水甘油酰肼材料(hyperpolymer-assisted hydrazide functionalized microspheres,HHMs)可用于低丰度糖蛋白/糖肽的富集。
[Abstract]:Aim: to immobilize the hyperbranched polymer onto an amino silica sphere to form a novel glycopeptide enrichment material for the efficient separation and enrichment of glycopeptide. Methods: hyperbranched polyglycidyl was synthesized by anion ring-opening polymerization. Hyperbranched polyglycerol-aldehydesy (HPG-ALD) was oxidized by periodate to form hyperbranched polyglycerol-aldehydes.Then the hyperbranched polyglycerol-aldehydes-HPG-ALD was immobilized on the silica spheres. The glycopeptide was enriched by acyl hydrazide modified with dihydrazide adipate. Results: the material was successfully synthesized by scanning electron microscope (SEM), thermogravimetric analysis (TGA), scanning electron microscopy (SEM) and thermogravimetric analysis (TGA). The results show that hyperbranched glycidyl hydrazide (HHMs) can be used in the enrichment of low abundance glycoprotein / glycopeptide. Conclusion: hyperbranched glycidyl hydrazide (HHMs) can be used in the enrichment of low abundance glycoprotein / glycopeptide.
【作者单位】： 安徽医科大学;蛋白质组学国家重点实验室北京蛋白质组研究中心军事医学科学院放射与辐射医学研究所;
【基金】：国家重点基础研究“973”项目(2011CB910603) 国家高技术研究发展计划(2012AA020203) 北京市科技新星计划(Z121107002512014)资助项目
【分类号】：R318.08
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本文编号：1676815