透明质酸-明胶-纳米羟基磷灰石仿生骨支架的制备及成骨活性研究

发布时间：2018-03-28 18:10

本文选题：透明质酸　切入点：明胶　出处：《高校化学工程学报》2017年02期

【摘要】：采用冷冻干燥与化学接枝法制备了一种新型透明质酸(HA)-明胶(G)-纳米羟基磷灰石(nHAP)多孔复合支架,通过材料的物理表征和细胞培养实验考察了复合支架的生物相容性及成骨诱导能力。其中,利用扫描电镜(SEM)观察复合支架的表观形貌及支架上成骨前体细胞(MC3T3-E1)的生长状态,采用衰减全反射-傅里叶红外光谱(ATR-FTIR)检测支架成分,同时,对复合支架的物理参数进行表征,利用碱性磷酸酶(ALP)显色法检测复合支架的成骨诱导能力。结果显示,复合支架具备与骨组织相似的物理性能,MC3T3-E1细胞在支架上具有良好的黏附、增殖能力;扫描电镜图片显示,细胞在支架表面及孔隙内部长势良好,大多数细胞能贯穿整个支架生长并伸出伪足使支架与细胞相互连接形成网状结构;成骨诱导培养基和复合支架协同作用,明显提高了MC3T3-E1细胞的骨分化能力。因此,HA-G-nHAP复合支架可作为骨组织工程支架材料,并应用于组织再生和功能重建。
[Abstract]:A new porous composite scaffold of hyaluronic acid (HA), gelatin, hydroxyapatite (HA) and nano-hydroxyapatite (HAP) was prepared by freeze-drying and chemical grafting method. The biocompatibility and osteogenic induction of composite scaffolds were investigated by physical characterization and cell culture experiments. The morphology of composite scaffolds and the growth state of MC3T3-E1 cells on scaffolds were observed by scanning electron microscopy (SEM). The composition of the composite scaffold was detected by attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR). The physical parameters of the composite scaffold were characterized and the osteogenic ability of the composite scaffold was detected by alkaline phosphatase (ALP) colorimetry. The composite scaffold has similar physical properties as bone tissue and MC3T3-E1 cells have good adhesion and proliferative ability on the scaffold, and the SEM images show that the cells grow well on the surface and inside the pores of the scaffold. Most cells can grow throughout the scaffold and extend pseudopodia so that the scaffold connects with cells to form a reticular structure. Therefore, HA-G-nHAP composite scaffolds can be used as scaffolds for bone tissue engineering, and can be used for tissue regeneration and functional reconstruction.
【作者单位】：中国科学院大连化学物理研究所生物技术研究部;
【基金】：国家自然科学基金(81573394) 科技部国际合作项目(2015DFA00740)
【分类号】：R318.08;R68

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