骨髓间充质干细胞复合Bio-oss修复兔颅骨缺损的组织学变化

发布时间：2018-04-03 08:41

  本文选题：骨髓　切入点：间质干细胞　出处：《中国组织工程研究》2017年13期

【摘要】：背景:目前有一些关于骨髓间充质干细胞复合异体骨、人工合成骨替代材料促进骨缺损修复的研究,但尚未见骨髓间充质干细胞复合Bio-oss用于兔颅骨缺损修复的报道。目的:观察骨髓间充质干细胞复合Bio-oss修复兔颅骨缺损的效果。方法:分离培养雄性兔骨髓间充质干细胞,将其作为种子细胞,在雌兔颅骨采用外直径6 mm的环形骨钻制造全层骨缺损3个。将96只雌性兔随机等分为4组,复合物组植入Bio-oss复合骨髓间充质干细胞,Bio-oss组单纯植入Bio-oss,骨髓间充质干细胞组单纯植入骨髓间充质干细胞,空白组不进行植入修复。每个组表面均覆盖引导组织再生膜。结果与结论:复合物组成骨效果比其他3组更佳,Bio-oss组成骨效果比骨髓间充质干细胞组和空白组更好,骨髓间充质干细胞组与空白组成骨效果接近。说明以骨髓间充质干细胞作为种子细胞,Bio-oss作为支架材料复合植入兔颅骨缺损区有明显的促进成骨作用,为临床治疗骨缺损提供新思路。
[Abstract]:Background: there have been some studies on bone marrow mesenchymal stem cells (BMSCs) combined with allogeneic bone, synthetic bone substitute materials to promote bone defect repair, but no reports of bone marrow mesenchymal stem cells (BMSCs) combined with Bio-oss for repairing rabbit skull defects have been reported.Objective: to observe the effect of bone marrow mesenchymal stem cells (BMSCs) combined with Bio-oss in repairing rabbit skull defect.Methods: male rabbit bone marrow mesenchymal stem cells were isolated and cultured as seed cells.96 female rabbits were randomly divided into 4 groups. The composite group was implanted with Bio-oss combined with bone marrow mesenchymal stem cells (BMSCs), the bone marrow mesenchymal stem cells (BMSCs) group was implanted with BMSCs only, and the blank group was not implanted with BMSCs.The surface of each group was covered with guided tissue regeneration membrane.Results and conclusion: the effect of composite bone composition was better than that of the other three groups. The effect of Bio-oss was better than that of bone marrow mesenchymal stem cells group and blank group. The effect of bone marrow mesenchymal stem cell group was similar to that of blank bone.It is concluded that the composite implantation of bone marrow mesenchymal stem cells (BMSCs) as seed cells and Bio-oss as scaffold material can obviously promote osteogenesis in rabbit skull defect and provide a new idea for clinical treatment of bone defect.
【作者单位】： 西南医科大学口腔医学院;解放军成都军区机关医院口腔科;西南医科大学附属口腔医院口腔颌面外科;
【基金】：解放军成都军区“十二五”面上项目(C14050)~~
【分类号】：R318.08;R68
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