三维打印羟基磷灰石晶须增强复合骨修复支架

发布时间：2018-04-04 23:37

本文选题：三维打印　切入点：羟基磷灰石晶须　出处：《无机材料学报》2017年08期

【摘要】：利用三维打印技术成功制备羟基磷灰石晶须(HAPw)增强的聚己内酯(PCL)复合骨修复支架。通过改变三维打印的挤出速度和挤出气压,使不同含量HAPw均能在PCL基材中一致排列并均匀分布。PCL支架的机械强度随HAPw含量增加显著提高,添加33wt%HAPw使PCL支架强度提升了高达3倍。此外,HAPw使PCL支架表面与水的接触角从近100o降低至约50o,有效改善了细胞表面粘附。经过体外人类骨髓间充质干细胞(h BMSC)在支架上的培养实验,发现添加HAPw的复合支架具有更好的生物相容性,能够有效促进h BMSC的增殖生长,且HAPw-PCL复合支架上细胞具有更高的碱性磷酸酶(ALP)活性和OCN、RUNX2等相关成骨基因表达,显示出h BMSCs向成骨方向更好的分化及成骨活性。
[Abstract]:Hydroxyapatite whisker (HAPW) reinforced PCL composite bone repair scaffold was successfully fabricated by three dimensional printing technique.By changing the extrusion speed and extrusion pressure of 3D printing, the mechanical strength of PCL scaffolds with different content of HAPw can be arranged uniformly and distributed uniformly in PCL substrate. The mechanical strength of PCL scaffolds increases significantly with the increase of HAPw content, and the strength of PCL scaffolds increases by 3 times with the addition of 33wt%HAPw.In addition, HAPW reduced the contact angle between water and surface of PCL scaffold from nearly 100o to about 50o. it effectively improved cell surface adhesion.The culture of human bone marrow mesenchymal stem cells (hBMSC) on the scaffold in vitro showed that the composite scaffold added with HAPw had better biocompatibility and could effectively promote the proliferation and growth of h BMSC.The cells on HAPw-PCL composite scaffold had higher alkaline phosphatase (ALP) activity and OCN-RUNX2 related osteogenic gene expression, which showed that h BMSCs had better differentiation and osteogenic activity in the direction of osteogenesis.
【作者单位】：上海理工大学材料科学与工程学院;上海交通大学附属第六人民医院骨外科;
【基金】：国家自然科学基金青年项目(51302170) 上海市教委科研创新项目(14YZ085)~~
【分类号】：R318.08

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