低能量近红外光治疗眼底病安全参数的研究

发布时间：2018-04-05 02:35

本文选题：低能量光疗　切入点：光生物调节作用　出处：《电子科技大学》2017年硕士论文

【摘要】：光生物调节作用(Photobiomodulation,PBM)也称为低水平(或低能量)激光(或光)治疗(low-level laser or light therapy,LLLT),今年是发现低能量的光照有光生物调节作用的第50年,由于低能量光疗的生物调节作用十分广泛,且有非侵入性的优势,所以广泛应用于临床研究中,包括减轻疼痛,加快伤口愈合,神经损伤修复与再生,增加组织血流量,改善焦虑抑郁状态,治疗帕金森,视网膜疾病等。然而在众多研究过程中,PBM仍巨有较大争议。主要存在两个问题:一,PBM的机制不被完全了解,二,在不同的治疗中具体的参数不统一,如波长,能量密度,功率密度,脉冲,治疗时机,治疗次数等。本论文基于第二个问题,以低能量近红外光(810nm半导体激光)在眼底病治疗的参数为主要的研究对象,在理解低能量激光各参数的基础上,对不同能量近红外光照射青紫蓝兔视网膜后所得实际能量的数据分析及视网膜各层细胞形态结构的观察研究,获得对视网膜相对安全的治疗参数;并设计动物近红外光LED眼治疗仪模型;从而为近红外光在临床眼底病治疗中提供理论数据,使其更安全地运用于临床治疗中。实验内容主要如下:1.摘取青紫蓝兔眼球,去除视神经正下方巩膜,置于透明容器中,用810nm激光垂直入射,测量不同能量激光经角膜到达视网膜的实际能量,得出810nm激光在眼球的衰减率;2.对健康青紫蓝兔行不同能量梯度(50mw,100mw,200mw)的810nm激光,3mm光斑照射视网膜,找出光凝阈值,照射后1周后行视网膜组织石蜡切片,观察不同能量近红外光对视网膜各层结构的影响。3.设计近红外光LED阵列用于动物实验。通过实验得出以下结果:1.810nm激光在眼内的衰减率为28-30%左右。2.810nm激光,50mw,3mm光斑,长脉冲模式10min照射对青紫蓝兔视网膜各层结构无损伤,为低能量近红外光治疗眼底病的安全参数。3.设计并制作出简易动物用近红外光LED阵列。
[Abstract]:Photobiomodulation (Photobiomodulation, PBM) (also known as low level or low energy laser (or light) treatment (low-level laser or light) therapy, LLLT), this year is found that low energy light light biomodulation of fiftieth years, due to the low energy phototherapy biomodulation is very extensive, and there is not invasive advantages, it is widely used in clinical studies, including relieving pain, accelerate wound healing, nerve repair and regeneration of tissue, increase blood flow, improve the treatment of anxiety and depression, Parkinson, retinal diseases. However in many research process, PBM is still a controversial giant. There are two main problems a PBM mechanism is not completely understood, two, in the different treatment of specific parameters is not uniform, such as wavelength, energy density, power density, pulse frequency, treatment time, treatment etc. this thesis is based on second issues, with low Near infrared energy (810nm laser) in the parameters of ocular fundus diseases as the main object of study, based on the understanding of the low energy laser parameters on the observation of different energy near infrared light pigmented retina after the actual energy data analysis and the retinal cell morphology, treatment parameters are relatively safe for the retina; and design animal near infrared LED eye treatment instrument model; near infrared light so as to provide theoretical data in clinical treatment of fundus diseases, make it more safe for clinical treatment. The main contents are as follows: 1. the removal of pigmented eye, remove the optic nerve below the sclera, placed in a transparent container, with 810nm the laser vertical incidence, the actual energy measurement of different laser energy through the cornea to the retina, the decay rate of 810nm laser in eye; 2. of healthy rabbits do not The same energy gradient (50MW, 100MW, 200MW) 810nm laser, 3mm laser spot retinal photocoagulation, find out the threshold, after 1 weeks of retinal tissue paraffin sections after irradiation at different energy near-infrared light effects on retinal layers structure of the.3. design of near infrared LED array used in animal experiments. The following experimental results were obtained: 1.810nm laser in the eye of the decay rate is about 28-30%.2.810nm laser, 50MW, 3mm spot, long pulse mode 10min irradiation damage to the pigmented retina structure, safety parameters of.3. design for low energy near-infrared fundus disease treated by near infrared light and produce LED array with a simple animal.

【学位授予单位】：电子科技大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R773;R454.2

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