氯化镧对氧化铝陶瓷颗粒诱导RAW264.7细胞炎性因子表达的影响

发布时间：2018-04-06 04:37

本文选题：氯化镧　切入点：氧化铝陶瓷颗粒　出处：《中国修复重建外科杂志》2014年08期

【摘要】：目的通过观察氯化镧对氧化铝陶瓷颗粒诱导小鼠RAW264.7细胞分泌炎性因子IL-1β、TNF-α的影响,探讨氧化铝陶瓷颗粒与无菌性炎症的关系以及氯化镧对其影响。方法体外培养小鼠RAW264.7细胞,根据培养液不同将实验分成4组:A组为细胞培养液,B组加入1 mg/mL氧化铝陶瓷颗粒悬液,C组加入1 mg/mL氧化铝陶瓷颗粒悬液和10μmol/L氯化镧溶液,D组加入10μmol/L氯化镧溶液。采用MTT法检测细胞生长情况,RT-PCR、ELISA及Western blot检测炎性因子IL-1β、TNF-α、NF-κB基因及蛋白表达。结果 MTT检测示,各组细胞活性差异无统计学意义(F=2.180,P=0.142)。RT-PCR检测示,B组IL-1β、TNF-α、NF-κB mRNA相对表达量显著高于其余3组(P0.05);D组各基因表达量低于A组(P0.05)。ELISA检测示,B组IL-1β和TNF-α含量显著高于其余3组(P0.05);D组显著低于A组(P0.05)。Western blot检测示,B组NF-κB蛋白表达量显著高于其余3组(P0.05)。结论氧化铝陶瓷颗粒可刺激巨噬细胞分泌IL-1β和TNF-α,而氯化镧可一定程度抑制巨噬细胞分泌IL-1β和TNF-α。
[Abstract]:Objective to observe the effect of lanthanum chloride on the secretion of inflammatory factor IL-1 尾 TNF- 伪 by RAW264.7 cells induced by alumina ceramic particles, and to explore the relationship between alumina ceramic particles and aseptic inflammation and the effect of lanthanum chloride on it.Methods Mouse RAW264.7 cells were cultured in vitro.According to the different culture medium, the experiment was divided into 4 groups: cell culture medium, group B, adding 1 mg/mL alumina ceramic particle suspension, group C, adding 1 mg/mL alumina ceramic particle suspension, and group D, adding 10 渭 mol/L lanthanum chloride solution to 10 渭 mol/L lanthanum chloride solution.The expression of IL-1 尾 -TNF- 伪 -NF- 魏 B gene and protein were detected by MTT assay and Western blot Elisa respectively.Results MTT showed that,The expression of NF- 魏 B protein in group B was significantly higher than that in group A (P 0.05). Western blot analysis showed that the expression of NF- 魏 B protein in group B was significantly higher than that in group B (P 0.05).Conclusion Alumina ceramic particles can stimulate the secretion of IL-1 尾 and TNF- 伪 by macrophages, while lanthanum chloride can inhibit the secretion of IL-1 尾 and TNF- 伪 by macrophages to some extent.
【作者单位】：南昌大学第一附属医院骨科;
【分类号】：R318.08

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