有序纤维的静电纺丝制备及其仿生矿化研究

发布时间：2018-04-07 15:18

本文选题：静电纺丝　切入点：仿生矿化　出处：《暨南大学》2012年硕士论文

【摘要】：骨组织是沿C轴取向生长的纳米羟基磷灰石（HAP）晶体沿着胶原纤维的平行方向有序聚集构成的有机-无机复合材料。在骨组织的修复过程中，胶原纤维起着骨质HAP形成的模板作用而非胶原蛋白（NCPs）则促使HAP成核，但这种机制尚未明确。本文采用自行研制的连续平行板电极静电纺丝技术构建有序的PCL/Gelatin纤维膜，采用SEM、FTIR、EDS及万能材料试验机，研究不同的工艺参数对纤维性能的影响。进而以这种纤维膜为模板，在自行研制的双扩散凝胶体系中，研究具有成骨活性的小分子β-GP和有序化的纤维及二者协同作用对仿生矿化的影响，采用XRD、FTIR、EDS、SEM、TEM及HRTEM，分析有序纤维模板和β-GP对矿化产物组成、结构和聚集状态的影响，探讨骨质HAP形成的可能机理，，并为骨组织工程支架和修复材料的设计和制备提高理论依据。研究结果表明： 1.采用自行研制的连续平行板电极静电纺丝收集装置，纤维的直径随着平行板电极间距离，纺丝电压的增大而减小；随着进料速率的增大而增大；而且接收距离对直径的影响不大。 2.有序纤维的直径小于无序纤维直径，有序PCL纤维拉伸强度为27.63±0.75MPa，比无序纤维的拉伸强度增大了232%，弹性模量略微增大，断裂伸长率明显降低。 3.在双扩散凝胶体系中，以PCL/Gelatin纤维为模板，在纤维表面发生矿化，其矿物组成主要为HAP和DCPD的混合物。β-GP的加入能更好的促进矿化，使得形成的矿物中HAP的含量相对增加。 4.在有序的纤维膜上矿化形成的矿物晶体成分与无序纤维膜上的矿物没有很大差别，都是由HAP和DCPD的混合物。但有序纤维上的形成的矿化物构成的“花簇”聚集体沿纤维有序排列。本论文的新颖之处就在于：采用自行研制的静电纺丝装置及双扩散凝胶系统，证实了矿化晶体的聚集体可在有序纤维模板上沿纤维有序排列。
[Abstract]:Bone tissue is an organic-inorganic composite formed by ordered aggregation of nano-hydroxyapatite (HAP) crystals along the parallel direction of collagen fibers.In the process of bone tissue repair, collagen fiber acts as a template for bone HAP formation, but not collagen protein (NCPs) promotes the nucleation of HAP, but this mechanism is not clear.In this paper, the continuous parallel plate electrode electrospinning technique was used to construct the ordered PCL/Gelatin fiber membrane. The influence of different process parameters on the fiber properties was studied by means of SEM-FTIREDS and universal material testing machine.Furthermore, using this fiber membrane as template, the effects of 尾 -GP and ordered fibers with osteogenic activity on biomimetic mineralization were studied in a self-developed double-diffusion gel system.The effects of ordered fiber template and 尾 -GP on the composition, structure and aggregation of mineralized products were analyzed by means of TEM and HRTEM. The possible mechanism of bone HAP formation was discussed, and the theoretical basis for the design and preparation of scaffolds and repair materials for bone tissue engineering was improved.The results show that:1.The fiber diameter decreases with the increase of the distance between the electrodes and the spinning voltage, and increases with the increase of feed rate.Moreover, the receiving distance has little effect on the diameter.2.The tensile strength of ordered PCL fiber is 27.63 卤0.75MPa, which is 232mm higher than that of disordered fiber. The elastic modulus increases slightly and the elongation at break decreases obviously.3.In the double diffusion gel system, the PCL/Gelatin fiber was used as the template and the mineral composition was mainly composed of the mixture of HAP and DCPD. The addition of 尾 -GP could promote the mineralization better and increase the content of HAP in the formed minerals.4.The composition of mineral crystals formed by mineralization on the ordered fibrous membrane is not much different from that on the disordered fibrous membrane and is composed of a mixture of HAP and DCPD.But the "flower clusters" formed by the mineralized matter on the ordered fibers were arranged in order along the fibers.The novelty of this thesis lies in the fact that the aggregates of mineralized crystals can be arranged in an ordered fiber template by using a self-developed electrostatic spinning device and a double diffusion gel system.
【学位授予单位】：暨南大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.08

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