新型温度敏感水凝胶作为动脉瘤栓塞材料的初步研究

发布时间：2018-04-10 23:27

本文选题：微球 + 壳聚糖　；参考：《东南大学学报(医学版)》2016年01期

【摘要】：目的:探讨微球-壳聚糖/甘油磷酸钠水凝胶作为一种新型动脉瘤栓塞材料的可行性。方法:将壳聚糖/甘油磷酸钠溶液(1∶7)与海藻酸钠微球按不同比例混合,超声乳化、冰浴搅拌后,制成微球-壳聚糖/甘油磷酸钠水凝胶,检测其p H值、成胶时间和力学强度,选择最佳配比;通过体外动脉瘤模型栓塞实验评价其导管内操控性和瘤内成胶性,通过兔耳中动脉栓塞实验评价其组织相容性。结果:当壳聚糖/甘油磷酸钠溶液与海藻酸钠微球体积比为1∶2时,所得水凝胶的p H值为7.28,成胶时间约2.5 min,最大应力在压缩应变为20%时已达到14 k Pa,峰值可达25 k Pa。体外动脉瘤模型栓塞过程中未出现溢胶现象,拔除微导管时亦未见有黏管或拉丝现象。兔耳中动脉栓塞后未出现组织排异反应;3 d后血管壁见少量炎症细胞浸润,1周时炎症反应达到高峰,3周后血管壁炎症细胞明显减少。结论:初步证实微球-壳聚糖/甘油磷酸钠水凝胶是一种新型非黏附性组织工程材料,具有合适的p H值和成胶时间,良好的力学强度、操控性、瘤内成胶性和组织相容性,可用于动脉瘤栓塞。
[Abstract]:Objective: to investigate the feasibility of microspheres-chitosan / sodium glycerophosphate hydrogel as a new embolization material for aneurysms.Methods: chitosan / sodium glycerophosphate solution (1: 7) and sodium alginate microspheres were mixed in different proportion, emulsified by phacoemulsification and stirred in ice bath. The pH value of microspheres / chitosan / sodium glycerophosphate hydrogel was determined.The gelation time and mechanical strength were chosen to select the best ratio. The intraductal maneuverability and intratumoral gelatinization were evaluated by the external aneurysm embolization experiment and the histocompatibility was evaluated by the rabbit middle ear artery embolization experiment.Results: when the volume ratio of chitosan / sodium glycerophosphate solution to sodium alginate microsphere was 1:2, the pH value of the hydrogel was 7.28, the gelation time was about 2.5 min, and the maximum stress reached 14 kPawhen the compression strain was 20 and the peak value was 25 kPa.There was no glue overflow during the embolization of the aneurysm model in vitro, and there was no adhesive tube or wire drawing when the microcatheter was removed.There was no tissue rejection after middle ear artery embolization. After 3 days, a small number of inflammatory cells infiltrated the vascular wall. After 1 week, the inflammatory reaction reached the peak and the inflammatory cells in the vascular wall decreased significantly after 3 weeks.Conclusion: microspheres / chitosan / sodium glycerophosphate hydrogel is a new non-adhesive tissue engineering material with suitable pH value and gelation time, good mechanical strength, maneuverability, intratumoral gelation and histocompatibility.It can be used for embolization of aneurysms.
【作者单位】：江苏省苏北人民医院神经外科;吉林大学附属第一医院神经外科;
【基金】：苏北人民医院院级基金资助项目(yzucms201323)
【分类号】：R318.08

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