SPS制备多孔掺锶羟基磷灰石骨修复材料的生物活性实验研究

发布时间：2018-04-12 20:55

本文选题：掺锶羟基磷灰石 + 放电等离子烧结　；参考：《昆明理工大学》2017年硕士论文

【摘要】：本实验以碳酸氢铵作为造孔剂,在不使用粘结剂的前提下通过放电等离子烧结技术快速制备多孔纯羟基磷灰石和掺锶羟基磷灰石材料(掺锶量为5wt%)。XRD图谱表明烧结后掺锶羟基磷灰石是具有与羟基磷灰石相同结构的晶体,锶的掺入未生成其它杂相。扫描电镜显示各材料在烧结过程中均形成了 200μm左右的孔隙,孔隙率约为40%,孔与孔之间相互贯通,能够提供骨组织和血管等充足的长入空间。力学性能测试表明两种材料的压缩强度均在3MPa以上,接近于人松质骨的压缩强度,另外,锶的掺入提高了羟基磷灰石的力学性能。LDH活性检测表明两种材料均无细胞毒性。兔P3代骨髓间充质干细胞共培养实验表明羟基磷灰石掺入锶有助于促进骨髓间充质干细胞的增殖、成骨分化和黏附。成骨细胞MG-63共培养实验表明羟基磷灰石掺入锶有助于促进成骨细胞的增殖、分化、黏附和迁移。兔桡骨骨缺损修复实验表明掺锶羟基磷灰石具有良好的骨修复能力,植入8周后材料与宿主骨发生骨整合,材料表面大量编织骨生成,16周后材料基本降解,板状骨塑形,骨重塑基本完成。体内外实验表明多孔掺锶羟基磷灰石材料具有无细胞毒性、良好的骨传导性和生物降解性,是一种理想的骨缺损修复材料。
[Abstract]:In this experiment, ammonium bicarbonate was used as pore-forming agent.Rapid preparation of porous pure hydroxyapatite and strontium doped hydroxyapatite by spark plasma sintering without binder (strontium content is 5wt%).XRD diagram shows that strontium doped hydroxyapatite is associated with hydroxyl group).Apatite crystals of the same structure,The incorporation of strontium did not produce other heterogenous phases.Scanning electron microscopy (SEM) showed that about 200 渭 m pores were formed in the sintering process, and the porosity was about 40 渭 m. The porosity was about 40 渭 m, and the holes were interpenetrating with each other, which could provide sufficient long space for bone tissue and blood vessels.Mechanical properties test showed that the compressive strength of the two materials was above 3MPa, close to the compressive strength of human cancellous bone. In addition, the incorporation of strontium enhanced the mechanical properties of hydroxyapatite. The results showed that the two materials had no cytotoxicity.The co-culture of bone marrow mesenchymal stem cells of rabbit P3 generation showed that the incorporation of hydroxyapatite with strontium could promote the proliferation, osteogenic differentiation and adhesion of bone marrow mesenchymal stem cells.The co-culture of osteoblasts by MG-63 showed that the incorporation of strontium into hydroxyapatite could promote the proliferation, differentiation, adhesion and migration of osteoblasts.The repair experiment of rabbit radius bone defect showed that strontium doped hydroxyapatite had good bone repair ability. After 8 weeks of implantation, the material was integrated with the host bone, the material was basically degraded after 16 weeks of formation of a large number of braided bone on the surface of the material, and the plate bone was shaped.Bone remodeling was basically completed.In vitro and in vivo experiments showed that the porous strontium doped hydroxyapatite material had no cytotoxicity, good bone conduction and biodegradability, and was an ideal bone defect repair material.
【学位授予单位】：昆明理工大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R318.08

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