花粉与P123双模板制备的新型多级孔生物活性玻璃对MG63细胞增殖和分化的影响

发布时间：2018-04-13 13:11

本文选题：新型多级孔生物活性玻璃 + MG细胞　；参考：《口腔医学研究》2017年09期

【摘要】：目的:检测花粉与P123双模板制备的新型多级孔生物活性玻璃对人前成骨细胞系MG63增殖及成骨分化的影响。方法:在材料工作浓度为0.2g/mL的浸提液(含10%胎牛血清的DMEM)中,培养MG63细胞至1、3、5d,分别采用MTT比色法检测细胞增殖水平;碱性磷酸酶试剂盒检测细胞内ALP活性;实时定量PCR法测定成骨标志性基因RUNX-2、SP7、COL-1的mRNA表达水平。结果:花粉与P123双模板制备的新型多级孔生物活性玻璃可促进MG63细胞增殖,增强ALP活性表达,上调MG63标志性基因RUNX-2、SP7、COL-1的mRNA表达水平。结论:以花粉与P123双模板制备的新型多级孔生物活性玻璃具有细胞毒性低,且具有促进成骨细胞成骨向分化的潜能。
[Abstract]:Aim: to investigate the effects of a new type of multiporous bioactive glass prepared by pollen and P123 double template on the proliferation and osteogenic differentiation of human proosteoblast cell line MG63.Methods: MG63 cells were cultured in the extractant containing 10% fetal bovine serum (0.2g/mL) for 5 days. The proliferation of cells was detected by MTT colorimetry, and the activity of ALP was detected by alkaline phosphatase assay kit.The expression of mRNA in the osteoblast iconic gene RUNX-2, SP7 and COL-1 was detected by real-time quantitative PCR.Results: the new multi-pore bioactive glass prepared by pollen and P123 double template could promote the proliferation of MG63 cells, enhance the expression of ALP activity, and up-regulate the mRNA expression level of MG63 iconic gene RUNX-2G SP7COL-1.Conclusion: the new multipore bioactive glass prepared by pollen and P123 has low cytotoxicity and the potential to promote osteoblast osteogenesis differentiation.
【作者单位】：吉林大学口腔医学院正畸科吉林省牙发育及颌骨重塑与再生重点实验室;吉林大学口腔医学院牙周病科;吉林大学食品科学与工程学院;
【基金】：国家自然科学基金面上项目(编号:81371153) 吉林省科技厅自然科学基金项目(编号:20150101173JC) 白求恩前沿交叉学科创新项目(编号:20131080031)
【分类号】：R783.1

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