利用罗曼鸡肌腱滑液鞘体内构建趾深屈肌腱的生物力学及组织学研究

发布时间：2018-04-18 06:16

本文选题：肌腱 + 腱鞘　；参考：《医用生物力学》2014年03期

【摘要】：目的探索肌腱滑液鞘对肌腱体内再生的作用。方法将36只罗曼鸡随机平均分为A、B两组。A组把左趾深屈肌腱滑液鞘的上、下、右侧分离,不切断趾深屈肌腱。同种异体脱细胞肌腱用部分分离的腱滑液鞘包裹,并固定在趾深屈肌腱左侧。B组直接把同种异体脱细胞肌腱固定在去除肌腱滑液鞘的趾深屈肌腱左侧。另取右趾深屈肌腱作为正常对照组。分别在第4、8、12周取材进行趾深屈肌腱最大载荷、弹性模量的生物力学检测及HE染色的组织学检测。结果术后第4、8、12周时,除第4周A、B组在弹性模量方面差异无统计学意义,其余时间点A组最大载荷和弹性模量都大于B组(P0.05)。随时间延长,A组胶原纤维增多,排列紧密,方向一致,炎性细胞浸润及纤维组织增生程度较轻。而B组胶原纤维数量逐渐减少、排列逐渐散乱,炎性细胞浸润、纤维组织增生程度明显高于A组。结论腱滑液鞘有助于肌腱再生,说明合适的体内环境对肌腱构建具有重要作用,本研究结果对临床上肌腱缺损病人找到合适肌腱替代物有积极作用。
[Abstract]:Objective to explore the effect of synovial sheath on tendon regeneration in vivo.Methods Thirty-six Rohmann chickens were randomly divided into two groups: group A and group A. the synovial sheath of the left deep flexor tendon was separated from the upper, lower and right sides, and the tendon of the deep flexor digitorum was not transected.The allogeneic acellular tendon was wrapped in the synovial sheath of partially separated tendon and fixed on the left side of flexor digitorum tendon. Group B directly fixed the allogeneic acellular tendon on the left side of flexor digitorum tendon which removed the sheath of synovial fluid of tendon.The flexor tendon of right toe was taken as the normal control group.The maximum load, elastic modulus of flexor digitorum flexor tendon and histological examination of HE staining were measured at the 12th week of the 4th week (8 ~ (th) ~ (th) ~ (th) ~ (th) ~ (th) ~ (th) week).Results at 12 weeks after operation, there was no significant difference in elastic modulus between group A and group B except at week 4. The maximum load and modulus of elasticity in group A were higher than those in group B (P 0.05).In group A, the collagen fibers increased, arranged closely and in the same direction, and the degree of inflammatory cell infiltration and fibrous tissue proliferation was slight.However, the number of collagen fibers in group B was gradually decreased, the arrangement was gradually scattered, inflammatory cells infiltrated, and the degree of fibrous tissue proliferation was significantly higher than that in group A.Conclusion the synovial sheath of tendon is helpful to tendon regeneration, which indicates that proper internal environment plays an important role in tendon construction. The results of this study have a positive effect on finding suitable tendon replacement in clinical patients with tendon defect.
【作者单位】：上海交通大学附属第九人民医院整复外科;
【分类号】：R318.08

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