多光子显微技术用于人体组织诊断的研究

发布时间：2018-04-18 21:06

本文选题：多光子显微术 + 双光子激发荧光　；参考：《福建师范大学》2012年硕士论文

【摘要】：基于双光子激发荧光(TPEF)和二次谐波(SHG)成像的多光子显微技术(MPM),能够同时清晰的呈现细胞种类和细胞外基质成分,在人体组织的无损诊断中它将成为一个重要的诊断工具。因为多光子显微成像技术拥有较深的成像穿透深度,较少的失焦光漂白和较低的光毒性,这使它更适合用来做活体研究。本论文旨在将该技术用于人体皮肤真皮、结肠的黏膜下层和胃组织的研究。通过系统分析,观测并研究不同组织胶原和弹力纤维成分的微观结构,从胶原和弹力纤维的形态特征的变化等要素中获得了评价区分病变的和正常的人体组织的指标。研究表明基于双光子激发荧光和二次谐波成像的多光子显微技术在胃、肠的黏膜组织和人体的皮肤组织疾病的早期诊断和治疗中有着极其重大的应用价值,可提供一种实时无损的活体诊断方法。
[Abstract]:Multiphoton microscopy based on two-photon excited fluorescence (TPEF) and second harmonic SHG imaging can clearly present cell types and extracellular matrix components at the same time. It will become an important diagnostic tool in the nondestructive diagnosis of human tissues.Because multiphoton microimaging technology has deeper imaging penetration depth less defocus bleaching and lower phototoxicity it is more suitable for in vivo research.The purpose of this study was to apply this technique to the study of submucous and gastric tissues in human dermis, colon and colon.Through systematic analysis, the microstructure of collagen and elastic fiber components in different tissues were observed and studied. From the changes of morphological characteristics of collagen and elastic fibers, the indexes to evaluate and distinguish pathological and normal human tissues were obtained.It has been shown that multiphoton microscopy based on two-photon excitation fluorescence and second-harmonic imaging is of great value in the early diagnosis and treatment of gastric and intestinal mucosal and human skin tissue diseases.A real-time and non-destructive method for living diagnosis can be provided.
【学位授予单位】：福建师范大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.51

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