无细胞支架修复家兔关节软骨缺损的实验研究

发布时间：2018-04-19 16:35

本文选题：软骨损伤、缺损 + 支架　；参考：《中国矫形外科杂志》2015年14期

【摘要】：[目的]探讨无细胞的软骨修复支架对动物模型关节软骨损伤的修复效果,及其临床应用的可能性和优势。[方法]成年新西兰大白兔构建软骨缺损动物模型。A组为空白对照组,仅做缺损手术;B组为无细胞支架组,植入不含细胞的支架;C组为负载细胞的支架组,植入负载种子细胞的支架。术后6、12周取材,体视显微镜观察、HE染色和甲苯胺蓝染色观察。术后12周的标本参考国际软骨修复协会组织学评分(International Cartilage Repair Society Histological Scoring,ICRS)标准评分,并对修复后新生组织力学性能进行检测。[结果]体视显微镜观察显示:A组,缺损周边仅有少量修复。B、C组,修复平面略低于周边正常组织,关节面较光滑,修复组织与周围软骨整合较好。HE染色与甲苯胺蓝染色显示:A组,缺损区域主要为纤维组织。B、C组,缺损区域中有大量软骨样组织生成。B、C两组评分的差别无统计学意义(P0.05),但两组的评分均显著高于A组(P0.05)。生物力学检测结果显示,正常软骨组织压缩模量为(0.61±0.06)MPa,A、B、C三组压缩模量分别为(0.08±0.05)MPa、(0.16±0.06)MPa、(0.22±0.06)MPa。与A组相比,B组与C组的压缩模量均有显著改善,但与正常软骨组织相比仍有明显降低(P0.05)。[结论]单纯支架植入与支架负载细胞共同植入,对关节软骨的修复效果无显著性差异,提示负载细胞并未对软骨修复效果有明显的改善。因此,本研究基于无需细胞种植的软骨再生技术理念,使用无细胞支架修复关节软骨损伤/缺损,有可能作为一种更为简便的再生医学方法在软骨损伤、缺损的治疗中发挥重要作用。
[Abstract]:[objective] to investigate the effect of acellular cartilage repair scaffold on articular cartilage injury in animal model and its clinical application.[methods] the adult New Zealand white rabbits were used as blank control group, group B as acellular scaffold group, group C as scaffold group with no cells implanted, and group A as blank control group.Implant a scaffold loaded with seed cells.He staining and toluidine blue staining were observed by stereoscopic microscope at 6 ~ 12 weeks after operation.12 weeks after the operation, the specimens were evaluated with the International Cartilage Repair Society Histological coring Standard (ICRS) and the mechanical properties of the new tissues were measured.[results] the stereoscopic microscope showed that there was only a small amount of repair around the defect in group A. the repair plane was slightly lower than that of the surrounding normal tissue, and the articular surface was smooth.The results of HE staining and toluidine blue staining showed that the defect area was mainly fibrous tissue.There was no significant difference in the scores between the two groups, but the scores in both groups were significantly higher than those in group A (P 0.05).The results of biomechanical examination showed that the compression modulus of normal chondrocytes was 0. 61 卤0. 06 ~ 0. 06 ~ 0. 010 ~ 0. 08 卤0. 05 ~ 0. 05 ~ 0. 05 ~ 0.16 卤0. 06 ~ 0. 06 ~ 0. 22 卤0. 06 卤0. 06 MPA, respectively, in the three groups, the compression modulus of the three groups was 0. 08 卤0. 05.Compared with group A, the compression modulus of group B and group C were significantly improved, but the compression modulus of group B was significantly lower than that of normal chondrocytes.[conclusion] there was no significant difference in the repair effect of articular cartilage between scaffold and scaffold cells, suggesting that the effect of cartilage repair was not improved obviously by loaded cells.Therefore, based on the concept of regenerating cartilage without cell implantation, this study uses cell-free scaffolds to repair articular cartilage injury / defect, which may be used as a more convenient method of regenerative medicine in cartilage injury.The treatment of defects plays an important role.
【作者单位】：北京积水潭医院北京市创伤骨科研究所;泰安军分区第一干休所;
【基金】：首都卫生发展科研专项资助项目(编号:首发2011-1006-01)
【分类号】：R687;R318.08

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