抗结核药物复合支架的制备及其性能研究

发布时间：2018-04-21 03:10

本文选题：脊柱结核 + 抗结核药　；参考：《中国脊柱脊髓杂志》2014年03期

【摘要】：目的:制备抗结核药物复合支架,并观察其载药性能、药物释放性能和组织相容性。方法:应用乳酸-羟基乙酸共聚物(PLGA)、磷酸三钙(β-TCP)、异烟肼(INH),通过结合相分离/粒子沥滤法制备成复合药物支架。采用扫描电镜观察支架的形貌;测定支架的孔隙率;在体外测定支架的生物力学强度、载药率、包封率以及药物释放特性;将复合支架(PLGA/β-TCP/INH)埋入大鼠肌肉中,4周后取材固定、染色行组织切片观察其组织相容性。结果:PLGA/β-TCP/INH复合支架表面及内部呈均匀多孔状,孔隙分布较均匀,在大孔的周围布满了相互贯通的微孔,外形多为近似圆形,大孔直径约150~300μm,平均222±23μm,小孔直径约10μm;孔隙率为(86±3)%;抗压强度为1.93±0.65MPa,药物包封率为(66.73±2.65)%;在体外药物释放过程较平稳,其释放曲线较平滑;组织学检查显示埋入大鼠肌肉中4周支架周围组织正常,细胞无变性坏死。结论:PLGA/β-TCP/INH复合支架具有良好的孔隙率、力学强度、释药特性和组织相容性,有望在脊柱结核病灶清除术后利用其修复骨缺损的同时发挥局部抗结核治疗作用。
[Abstract]:Aim: to prepare an anti-tuberculosis drug composite stent and to observe its drug loading, drug release and histocompatibility. Methods: the composite drug stents were prepared by the combination phase separation / particle leaching method with lactic acid-glycolic acid copolymers (PLGAA), tricalcium phosphate (尾 -TCPN) and isoniazid (INHH). The morphology of the scaffold was observed by scanning electron microscope; the porosity of the scaffold was measured; the biomechanical strength, drug loading rate, encapsulation efficiency and drug release characteristics of the scaffold were measured in vitro; and the composite scaffold PLGA / 尾 -TCP-INH was implanted into the muscle of rats for 4 weeks and fixed. Histocompatibility was observed by staining tissue sections. Results the surface and interior of the composite scaffold were homogeneous and porous, and the pores were evenly distributed. The pores were filled with interpenetrating micropores around the macropores, and most of them were approximately circular in shape. The pore diameter was about 150 ~ 300 渭 m (mean 222 卤23 渭 m), the pore diameter was 10 渭 m, the porosity was 86 卤3, the compressive strength was 1.93 卤0.65 MPA, the drug encapsulation rate was 66.73 卤2.65 渭 m, the release process of the drug in vitro was relatively stable, and the release curve was smooth. Histological examination showed that the tissue around the scaffold was normal and no degeneration and necrosis. Conclusion the composite scaffold of 尾 -PLGA / 尾 -TCP-INH has good porosity, mechanical strength, drug release characteristics and histocompatibility. It is expected to play a role in local antituberculotic therapy while repairing bone defects after focal clearance of spinal tuberculosis.
【作者单位】：山西医科大学;解放军第309医院全军骨科中心;中国科学院化学研究所;
【基金】：国家自然科学基金资助项目(编号:81071454)
【分类号】：R318.08

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