静电纺丝庆大霉素涂层钛板的制备及其体外性能研究

发布时间：2018-04-21 08:38

本文选题：静电纺丝 + 庆大霉素　；参考：《南京医科大学》2012年硕士论文

【摘要】：目的采用静电纺丝技术制备负载庆大霉素的钛板，并研究其做为局部释药系统在体外的一系列性能。内植物相关性感染是临床上非常棘手的问题，局部抗生素释放系统由于其较高的局部药物浓度，较少的全身毒性反应等优点在内植物相关性感染的防治方面受到越来越多的关注，本实验的目的是制备负载庆大霉素的抗生素涂层钛板并评价其体外性能。方法（1）采用静电纺丝技术在钛板表面制备负载庆大霉素的抗生素涂层（2）应用扫描电镜观察静电纺丝庆大霉素涂层钛板的表面微观结构；计算每块钛板表面负载的庆大霉素含量，研究静电纺丝庆大霉素涂层钛板体外释药行为（3）以裸钛板及不含庆大霉素的聚合物涂层钛板为空白组和对照组，以体外抑菌圈直径为主要考察指标研究静电纺丝庆大霉素涂层钛板体外抑制金黄色葡萄球菌的作用，并研究其体外抗金黄色葡萄球菌的粘附作用。（4）采用CCK-8法，以大鼠MC3T3-E1成骨细胞为效应细胞，研究静电纺丝庆大霉素涂层对鼠成骨细胞活力的影响。结果（1）扫描电镜下静电纺丝庆大霉素涂层钛板表面呈现为横断面为圆形的表面光滑的纳米纤维，纤维直径为705±122nm。每块钛板表面的庆大霉素平均含量为392.2±57.2μg。静电纺丝庆大霉素涂层钛板的体外药物释放表现为初始24h内的突释状态，随之为缓慢平稳的释放，持续2周左右，最终的释放量为71%，约278.3μg（2）静电纺丝庆大霉素涂层钛板在体外对金黄色葡萄球菌具有明显的抑制作用，，且体外抗菌作用可持续一周，其抗金黄色葡萄球菌粘附作用与裸钛板相比较具有统计学差异（p0.05）。（3）CCK-8实验结果显示静电纺丝庆大霉素涂层对比空白组能够促进鼠成骨细胞的粘附增殖，两组之间有统计学差异（p0.05），未见毒性反应。结论采用静电纺丝技术制备庆大霉素涂层钛板，技术要求简单方便经济，可在实验室或工厂中完成；在体外的释药行为研究中表现出可持续释药的特点；体外能够显著抑制金黄色葡萄球菌的粘附及生长；由于其表面的纳米纤维结构具有高比表面积及高孔隙率的特点，能够显著促进鼠成骨细胞的粘附与增殖，未见毒性反应。局部抗生素释放系统是防治内植物相关性感染的研究热点，采用静电纺丝这种新方法制备的庆大霉素涂层钛板具有良好的体外性能，在防治内植物相关性感染方面具有较大的潜力。
[Abstract]:Aim to prepare gentamicin loaded titanium plate by electrostatic spinning and study its properties as a local drug delivery system in vitro. Intra plant-associated infection is a very difficult clinical problem. The local antibiotic release system, due to its high local drug concentration, More and more attention has been paid to the prevention and treatment of plant-associated infection with less systemic toxicity. The aim of this study was to prepare antibiotic coated titanium plate loaded with gentamicin and evaluate its performance in vitro. Methods 1) Gentamycin loaded antibiotic coating was prepared on titanium plate by electrostatic spinning technique. SEM was used to observe the surface microstructure of gentamicin coated titanium plate, and the content of gentamycin loaded on each titanium plate was calculated. The in vitro drug release behavior of electrospun gentamicin coated titanium plate was studied. The naked titanium plate and the polymer coated titanium plate without gentamycin were used as blank group and control group. The inhibitory effect of gentamicin coated titanium plate on Staphylococcus aureus in vitro and its adhesion to Staphylococcus aureus in vitro were studied by CCK-8 method. The effect of electrospun gentamicin coating on the activity of rat osteoblasts was studied by using rat MC3T3-E1 osteoblasts as effector cells. Results 1) the surface of the electrospun gentamicin coated titanium plate showed a smooth surface with a circular cross section, and the diameter of the fiber was 705 卤122 nm under scanning electron microscope. The average content of gentamicin on each titanium plate was 392.2 卤57.2 渭 g. In vitro drug release of gentamicin coated titanium plate by electrospinning showed sudden release within 24 hours, followed by slow and steady release, which lasted for about 2 weeks. The final release of Gentamycin coated titanium plate was 71g about 278.3 渭 g 路g ~ 2) the electrospun gentamicin coated titanium plate had obvious inhibitory effect on Staphylococcus aureus in vitro, and the antibacterial effect could last for one week. The anti-staphylococcus aureus adhesion effect was significantly different from that of bare titanium plate. The results showed that the electrostatic spinning gentamicin coating could promote the adhesion and proliferation of rat osteoblasts compared with the blank group. There was a significant difference between the two groups (P 0.05), and no toxic reaction was found. Conclusion the technology of preparing gentamicin coated titanium plate by electrostatic spinning is simple, convenient and economical, which can be completed in laboratory or factory, and shows the characteristics of sustainable drug release in the study of drug release behavior in vitro. In vitro, the adhesion and growth of Staphylococcus aureus were significantly inhibited, and the nanofiber structure on its surface had the characteristics of high specific surface area and high porosity, which could significantly promote the adhesion and proliferation of rat osteoblasts, and no toxic reaction was found. Local antibiotic release system is a hot topic in the prevention and treatment of plant related infection. The gentamicin coated titanium plate prepared by electrostatic spinning has good performance in vitro. It has great potential in prevention and treatment of plant-related infections.
【学位授予单位】：南京医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.1

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