硅酸盐生物活性玻璃碎片杀菌的机制研究

发布时间：2018-04-21 18:09

本文选题：生物活性玻璃 + 抗菌作用　；参考：《华中师范大学》2012年硕士论文

【摘要】：当今,有众多的新型抗菌药物和材料被研究和应用。比如：光照TiO2杀菌、金属离子杀菌、季铵盐等等,它们的抗菌方式都属于纳米颗粒抗菌模式。生物活性玻璃就是其中的一种并且占有重要的地位。其拥有良好的生物活性、相容性、抗菌性,因此,拥有巨大的研究价值和广阔的应用前景。生物活性玻璃有42S,45S,S53P4、58S,77S等数种。而本文研究所选择代表性的45S5做为研究对象。它浸入液体环境中后,其表面发生一系列反应,最后在表面形成一层与人体骨骼相似的羟基磷灰石层,与人体组织细胞紧密结合。本文取45S5生物活性玻璃与大肠杆菌混合,进行平板菌落计数,测定其抗菌率,通过实验发现5min、15min、30min、60min四个时间段的抗菌率分别为54.55%、58.72%、42.78%、55.95%,具有明显杀菌效果。然后用透射电镜观察其杀菌过程,发现45S5及其表面碎片与细菌细胞接触后对膜结构产生破坏作用,导致细菌死亡；或者45S5碎片进入细菌细胞内与细胞内物质作用,导致细菌死亡；通过观察还发现两者有同时作用的现象。
[Abstract]:Nowadays, many new antimicrobial agents and materials have been studied and applied. For example: illumination TiO2 sterilization, metal ion sterilization, quaternary ammonium salt and so on, their antimicrobial methods belong to nanoparticles antibacterial mode. Bioactive glass is one of them and plays an important role. Because of its good bioactivity, compatibility and antibacterial properties, it has great research value and wide application prospect. There are several kinds of bioactive glass, such as 42SU 45SN S53P4C58S77S and so on. The representative 45S5 is chosen as the research object in this paper. After it was immersed in liquid environment, a series of reactions took place on its surface. Finally, a hydroxyapatite layer similar to human skeleton was formed on the surface, which combined closely with human tissue and cells. In this paper, 45S5 bioactive glass was mixed with Escherichia coli, and its antibacterial rate was determined by plate colony count. It was found that the antibacterial rate was 54.55% and 58.72% in four periods of 5 min ~ 15 min ~ 30 min ~ 60 min ~ 60 min, respectively, and the bactericidal effect was obvious. Then the bactericidal process of 45S5 and its surface fragments were observed by transmission electron microscopy. It was found that 45S5 and its surface fragments had a destructive effect on the membrane structure after contact with bacterial cells, leading to the death of bacteria, or that 45S5 fragments enter bacteria cells and interact with intracellular substances. Causes the bacteria to die; through the observation also discovered the both to have simultaneously the phenomenon.
【学位授予单位】：华中师范大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.08

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