散射成像式流式细胞仪液流系统设计与探究

发布时间：2018-04-23 07:42

本文选题：流式细胞仪 + 低流速　；参考：《天津大学》2012年硕士论文

【摘要】：流式细胞仪作为一种新型的细胞定量分析和分选仪器，能够在功能层面上对单个细胞进行快速多参数分析，但其形态学信息检测能力略显不足。本课题组研究的散射成像式流式细胞仪正好弥补这一不足，该系统基于成像原理，用CCD记录与细胞形态信息相关的散射图像。实验证明利用获得的散射图像能够识别淋巴B细胞与T细胞，对细胞形态学研究具有重要意义，然而受相机快门速度的限制，亟需开发一种适合成像的低流速液流系统。本课题正是基于这一目的从流体室设计、液流驱动系统设计以及鞘流压强PID控制三个方面进行深入研究。 1、流体室是流式细胞仪的核心部件，，对仪器的功能实现具有重大影响，本论文按照流式细胞仪流动单细胞测量的特点，进行了流体室设计、流体运动状态分析、最佳检测区位置选择，并结合COMSOL Mutiphysics软件模拟结果与实验效果，对流体室结构进行改进。 2、液流驱动系统是液流系统的重要组成部分，其性能直接影响流体室内核流稳定性。在系统开发过程中先后设计并试验多种驱动方案，包括重力、针管泵、压强等多种形式，并在大量实验基础上，综合考虑核流形状稳定性、压缩直径、流速等因素，最终确定气压驱动鞘流、针管泵驱动核流的方案。 3、论文设计了以单片机、注气泵、压强传感器构成反馈回路的数字PID闭环负反馈系统，并进行参数整定。实验结果显示：该低流速液流系统能够在流速低至5mm/s时，将核流直径压缩到30μm以下，且核流形状稳定，能够满足散射成像式流式细胞仪的成像需求。
[Abstract]:As a new type of cell quantitative analysis and sorting instrument, flow cytometry (FCM) can analyze a single cell quickly and multi-parameter on the functional level, but its ability of morphological information detection is a little insufficient. The scattering imaging flow cytometry (FCM) studied by our team can make up for this deficiency. Based on the imaging principle, the system records scattering images related to cell morphological information by CCD. Experimental results show that the scattering images can recognize lymphoid B cells and T cells, which is of great significance to the study of cell morphology. However, due to the limitation of camera shutter velocity, it is urgent to develop a low velocity liquid flow system suitable for imaging. The purpose of this paper is to study the design of fluid chamber, the design of fluid flow drive system and the PID control of sheath flow pressure. 1. The fluid chamber is the core component of the flow cytometry, which has great influence on the realization of the function of the instrument. According to the characteristics of flow cytometry single cell measurement, the fluid chamber is designed and the fluid motion state is analyzed. The structure of the fluid chamber was improved by selecting the best location of the detection area and combining the simulation results with the experimental results of COMSOL Mutiphysics software. 2. Liquid flow drive system is an important part of liquid flow system, and its performance directly affects the stability of nuclear flow in fluid chamber. In the course of system development, many kinds of driving schemes were designed and tested successively, including gravity, needle pump, pressure and so on. On the basis of a large number of experiments, the stability of nuclear flow shape, compression diameter, flow rate and other factors were considered synthetically. Finally, the scheme of pneumatic driven sheath flow and needle pump driven nuclear flow was determined. 3. A digital PID closed loop negative feedback system composed of single chip microcomputer, air pump and pressure sensor is designed, and the parameters are adjusted. The experimental results show that the low velocity liquid flow system can compress the diameter of the nuclear flow below 30 渭 m when the flow velocity is lower than 5mm/s, and the shape of the nuclear flow is stable, which can meet the imaging requirements of scattering imaging flow cytometry.
【学位授予单位】：天津大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.6

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