软骨细胞及骨髓间充质干细胞共培养修复关节软骨缺损

发布时间：2018-04-26 21:10

  本文选题：软骨细胞 + 间质干细胞　； 参考：《中国组织工程研究》2017年32期

【摘要】：背景:关节软骨损伤多由创伤及骨关节炎引起,其治疗是临床研究的难点和热点。组织工程技术的发展为修复关节软骨缺损提供了新方法,但软骨细胞来源不足一直是一个难题。目的:探讨软骨细胞及骨髓间充质干细胞共培养修复关节软骨缺损的可行性,评价修复效果。方法:体外培养扩增猪骨髓间充质干细胞及关节软骨细胞,共培养2代,接种于聚乳酸-聚羟基乙酸聚合物上继续共培养2周,构建共培养组织工程软骨(共培养组);以单纯软骨细胞构建组织工程软骨为对照组,回植修复关节软骨缺损;空白组软骨缺损不作处理。半年后行大体观察及组织学观察,定量检测Ⅱ型胶原染色面积及氨基糖胺聚糖含量。结果与结论:(1)共培养组的修复组织呈软骨样,表面光滑平坦,与周围关节软骨组织整合良好;对照组呈纤维组织修复;而空白组则无修复;(2)组织学观察显示,共培养组结构致密,细胞外基质分布更均匀,与周围关节软骨组织及软骨下骨组织整合良好;(3)共培养组的Ⅱ型胶原染色面积及糖胺聚糖含量均优于对照组及空白组,差异有显著性意义(P0.05);(4)综上,软骨细胞和骨髓间充质干细胞共培养构建组织工程软骨能有效修复关节软骨缺损。
[Abstract]:Background: articular cartilage injury is caused by trauma and osteoarthritis. The development of tissue engineering provides a new method for repairing articular cartilage defects, but the shortage of chondrocytes is always a difficult problem. Aim: to investigate the feasibility of co-culture of chondrocytes and bone marrow mesenchymal stem cells (BMSCs) for repair of articular cartilage defects. Methods: porcine bone marrow mesenchymal stem cells and articular chondrocytes were cultured in vitro. Co-culture of tissue engineered cartilage (co-cultured group); using pure chondrocyte to construct tissue engineered cartilage as control group and replantation to repair articular cartilage defect; the blank group was not treated with cartilage defect. Gross and histological observation were performed half a year later. The staining area and aminoglycosaminoglycan content of type 鈪，

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