机械法准分子激光上皮瓣下角膜磨镶术后早期泪液中肝细胞生长因子含量与角膜上皮下雾状混浊的相关性

发布时间：2018-04-28 15:22

本文选题：Epi-LASIK + 泪液　；参考：《南方医科大学学报》2017年11期

【摘要】：目的观察兔眼机械法准分子激光上皮瓣下角膜磨镶术(Epi-LASIK)术后早期泪液中肝细胞生长因子(HGF)含量的动态变化及其与角膜上皮下雾状混浊(雾状混浊)的关系。方法 24只(48眼)新西兰白兔,双眼分别建立切削深度100μm Epi-LASIK和切削深度150μm Epi-LASIK动物模型。采用酶联免疫反应吸附实验双抗体夹心法测定两组术前、术后3、7、14、30 d泪液中HGF的水平,并观察两组术后雾状混浊的情况。结果不同切削深度两组Epi-LASIK术后角膜上皮均于3~5 d愈合。两组均于术后3 d开始形成雾状混浊,伴随泪液中HGF含量的升高。150μm切削深度组术后3、7、14、30 d雾状混浊程度较100μm组明显(P0.05),且泪液中HGF的水平明显高于100μm组(P0.05)。结论两组Epi-LASIK术后早期泪液中HGF与雾状混浊的程度表现为正相关,且与切削深度相关。
[Abstract]:Objective to observe the dynamic changes of HGF- HGF in tears of rabbit eyes after Epi-LASIKectomy with mechanical excimer laser subepithelial flap and its relationship with haze opacity (fog opacity) of cornea. Methods Twenty four New Zealand white rabbits were used to establish the animal models of cutting depth of 100 渭 m Epi-LASIK and cutting depth of 150 渭 m Epi-LASIK in both eyes. The levels of HGF in tears of the two groups were measured by Elisa double antibody sandwich method on the 30th day after operation, and the fog opacity was observed in the two groups. Results Corneal epithelium healed at 3 ~ 5 days after Epi-LASIK in two groups with different cutting depth. Fog opacity began to form in both groups on the 3rd day after operation. With the increase of HGF content in tear. 150 渭 m cutting depth, the degree of fog turbidity was significantly higher than that of 100 渭 m group on the 3rd day after operation (P 0.05), and the level of HGF in tear was significantly higher than that in 100 渭 m group (P 0.05). Conclusion there was a positive correlation between HGF and fog turbidity in tear in both groups after Epi-LASIK, and there was a positive correlation between HGF and depth of ablation.
【作者单位】：广州军区广州总医院眼科;
【基金】：广东省医学科研基金(B2010249)
【分类号】：R779.63

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