生物表面活性剂诱导丝素蛋白水凝胶的研究

发布时间：2018-05-01 13:18

  本文选题：丝素蛋白 + 水凝胶　； 参考：《苏州大学》2015年硕士论文

【摘要】：丝素蛋白水凝胶基于自身良好的生物相容性,在生物材料方面具有潜在的应用。本文研究了三类表面活性剂,即甜菜碱类表面活性剂、多肽类表面活性剂和氨基酸类表面活性剂诱导丝素形成水凝胶,表征其结构和性能,并探究其凝胶化机理。甜菜碱类表面活性剂能加快丝素蛋白凝胶化,其凝胶化时间与活性剂碳链长度无关,而与表面张力有关,表面张力出现最低点的活性剂成凝胶时间较短,其中十二烷基二甲基甜菜碱诱导丝素形成凝胶时间较短,在20 min左右。对所形成的水凝胶结构和性能进行表征,并通过荧光测试其内部结构变化,这一过程中,在甜菜碱类表面活性剂的亲疏水作用下,丝素蛋白分子链伸张开并重组,丝素蛋白构象由无规线团向β-折叠结构转变,形成结构均匀,孔径较大的三维网络结构水凝胶,柔软而富有弹性,具有一定的抑菌作用,可作为水凝胶医用敷料,解决传统创口贴带来的诸多不便。多肽类表面活性剂枯草菌脂肽钠诱导丝素蛋白形成水凝胶,所需枯草菌脂肽钠浓度较低,37℃下1 g/L的枯草菌脂肽钠即可诱导丝素在2 h内形成水凝胶,且凝胶具有一定的细胞生物相容性。氨基酸类表面活性剂N-月桂酰肌氨酸钠(SNS)能够加速丝素蛋白形成水凝胶。SNS与丝素混合后,可通过温度、SNS浓度和丝素浓度的调节来满足所需胶凝时间。利用红外光谱、X射线衍射、扫描电镜、圆二色光谱和荧光光谱等对水凝胶的结构、性能和凝胶机理进行探究。发现SNS诱导丝素蛋白分子链在亲疏水力和静电作用下展开重构,形成更多的结晶结构交联成水凝胶。SNS/SF水凝胶结构稳定,具有相对较高的强度和优良的弹性,在适度应力作用下变形可回复,细胞生物相容性良好。SNS/SF水凝胶在组织修复方面具有巨大的潜力。
[Abstract]:Silk fibroin hydrogel has potential applications in biomaterials because of its good biocompatibility. In this paper, three kinds of surfactants, beet surfactants, polypeptide surfactants and amino acid surfactants, have been studied to induce silk fibroin to form hydrogels, to characterize their structure and properties, and to explore their gelation mechanism. Beet surfactants can accelerate the gelation of silk fibroin protein. The gelation time is not related to the length of the carbon chain of the surfactant, but to the surface tension. The gelation time of the surfactant with the lowest surface tension is shorter. The gelation time of silk fibroin induced by dodecyl dimethyl betaine was about 20 min. The structure and properties of the hydrogel were characterized, and its internal structure was measured by fluorescence. In this process, the silk fibroin molecular chain was stretched and recombined under the hydrophobic action of betaine surfactants. The conformation of silk fibroin is changed from random coil to 尾 -fold structure to form a three-dimensional hydrogel with uniform structure and large pore diameter. It is soft and elastic, has certain bacteriostatic effect, and can be used as hydrogel medical dressing. To solve the traditional band-paste brought many inconveniences. The polypeptide surfactant, sodium subtilis lipidopeptide, induced the formation of hydrogel of silk fibroin protein, and the concentration of sodium subtilis lipid peptide sodium was 1 g / L at 37 鈩，

本文编号：1829525