含铜不锈钢铜离子释放机制研究及其生物学效应探索

发布时间：2018-05-03 08:43

本文选题：含铜不锈钢 + 铜离子溶出　；参考：《辽宁科技大学》2014年硕士论文

【摘要】：在医用金属材料中，医用不锈钢具有优良的耐腐蚀性能、高强韧性、易加工和成本低等优点，成为目前临床上应用最广泛医用金属材料之一。然而，目前的医用不锈钢不具有生物活性。外科植入手术中细菌感染是导致手术失败的主要原因。随着人们对生活质量的要求越来越高，医用金属材料需要具有良好的抗菌性能。铜元素不但是人体必须的微量元素，还具有良好的杀菌性能。在普通不锈钢中添加适量的铜，通过热处理的方式使含铜不锈钢在使用时释放铜离子，使含铜不锈钢具有良好的生物功能和抗菌性能。本文对含铜不锈钢的铜离子释放机制和其生物功能进行了探索，得到了以下研究成果：（1）含铜不锈钢的铜离子溶出机制含铜不锈钢的表面钝化膜与普通不锈钢不同，铜离子参与钝化膜的形成，并随钝化膜的腐蚀而溶出。由于含铜不锈钢中析出富铜相，含铜不锈钢的耐点蚀性能略低于普通不锈钢，但是耐均匀腐蚀性与普通不锈钢相近。经过硝酸钝化后含铜不锈钢的耐点蚀性能与普通不锈钢相差无几。（2）含铜不锈钢的抗菌性能含铜不锈钢对生活中和外科植入手术造成感染的常见细菌：金黄色葡萄球菌和大肠杆菌，具有良好的杀灭作用。含铜不锈钢对生物膜的形成也具有良好的抑制作用。硝酸钝化后含铜不锈钢的杀菌性能有所下降。通过用硫酸铜溶液代替不锈钢溶出的铜离子，得到铜离子的最低杀菌浓度(MBC)为50ppb。（4）含铜不锈钢的生物相容性含铜不锈钢的溶血率小于5%，不会发生溶血，表明铜元素的添加不会对不锈钢的血液相容性造成影响。生物安全性评价说明317L-4.5Cu不锈钢没有细胞毒性，符合植入标准。
[Abstract]:Among the medical metal materials, medical stainless steel has the advantages of excellent corrosion resistance, high strength and toughness, easy processing and low cost, so it has become one of the most widely used medical metal materials in clinic. However, the current medical stainless steel does not have biological activity. Bacterial infection in surgical implantation is the main cause of failure. With the increasing demand for quality of life, medical metal materials need to have good antibacterial properties. Copper is not only a necessary trace element for human body, but also has good bactericidal performance. By adding proper amount of copper to common stainless steel, copper ion is released from copper containing stainless steel by heat treatment, which makes copper stainless steel have good biological function and antibacterial property. In this paper, the mechanism of copper ion release and its biological function of copper containing stainless steel have been explored, and the following research results have been obtained: Copper ion dissolution mechanism of copper containing stainless steel The surface passivation film of copper-containing stainless steel is different from that of common stainless steel. Copper ion takes part in the formation of passivation film and dissolves with the corrosion of passivation film. Due to the copper-rich phase in the copper-containing stainless steel, the pitting corrosion resistance of the copper-containing stainless steel is slightly lower than that of the common stainless steel, but the uniform corrosion resistance is similar to that of the common stainless steel. After passivation with nitric acid, the pitting corrosion resistance of copper containing stainless steel is similar to that of ordinary stainless steel. Antibacterial properties of copper containing stainless steel Copper-containing stainless steel has a good killing effect on common bacteria in life and surgical implantation: Staphylococcus aureus and Escherichia coli. Copper-containing stainless steel also has a good inhibitory effect on the formation of biofilm. The bactericidal properties of copper-containing stainless steel decreased after passivation with nitric acid. The minimum bactericidal concentration of copper ion (MBC) is 50 ppb by replacing the copper ion dissolved in stainless steel with copper sulfate solution. Biocompatibility of copper containing stainless steel The hemolysis rate of the stainless steel containing copper is less than 5 and the hemolysis will not occur, which indicates that the addition of copper does not affect the blood compatibility of the stainless steel. Biosafety evaluation shows that 317L-4.5Cu stainless steel is not cytotoxic and meets the implantation criteria.
【学位授予单位】：辽宁科技大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R318.08

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