脂肪干细胞与黄芪多糖胶原的生物相容性及其复合材料促进血管新生效果的实验研究

发布时间：2018-05-04 01:39

本文选题：脂肪干细胞 + 黄芪多糖胶原　；参考：《南京中医药大学》2013年硕士论文

【摘要】：研究目的： 1研究脂肪干细胞和黄芪多糖胶原复合材料的可行性和生物相容性。 2研究脂肪干细胞联合黄芪多糖胶原对血管新生的影响。 3探讨黄芪多糖胶原与脂肪干细胞之间的作用影响及可能存在的机制,评价该复合物的优劣、目前存在的问题以及在后续实验及临床研究中的意义研究方法： 1体外培养脂肪干细胞,观察其生物学特性。 2制备黄芪多糖胶原,检测胶原相关特性。 3将脂肪干细胞种植入黄芪多糖胶原,通过光镜观察、黏附率检测、细胞增殖等手段研究脂肪干细胞与黄芪多糖胶原的生物相容性。 4ELISA法检测共培养后,脂肪干细胞与黄芪多糖胶原复合物所分泌的VEGF含量。 5鸡胚尿囊膜实验检测脂肪干细胞与黄芪多糖胶原复合物在促进血管新生方面的效果。研究结果： 1脂肪干细胞的生物学研究：培养的脂肪干细胞形态良好,生长良好。 2脂肪干细胞与黄芪多糖胶原的生物相容性：脂肪干细胞可以在黄芪多糖胶原支架上黏附、生长和增殖,其黏附率为：%；脂肪干细胞在黄芪多糖胶原支架上能够正常生长和增殖,并且黄芪多糖胶原在一定的阶段内对脂肪干细胞的增殖有促进作用。 3黄芪多糖胶原和脂肪干细胞共培养时,对脂肪干细胞所分泌的细胞因子VEGF有促进作用。 4脂肪干细胞与黄芪多糖胶原复合物对鸡胚尿囊膜血管新生具有促进作用,并且明显高于对照组。研究结论：脂肪干细胞与黄芪多糖胶原具有良好的生物相容性,黄芪多糖胶原可以成为脂肪干细胞一个理想的支架载体。该复合材料具有促进血管新生的作用,其机制可能与脂肪干细胞分泌的细胞因子以及脂肪干细胞的横向分化相关。对于这方面可以有进一步的后续研究。
[Abstract]:Objectives of the study: 1 to study the feasibility and biocompatibility of the composite of Astragalus polysaccharide collagen and adipose stem cells. 2 to study the effect of adipose stem cells combined with Astragalus polysaccharide collagen on angiogenesis. 3 to explore the effect of Astragalus polysaccharides collagen on adipose stem cells and its possible mechanism, to evaluate the advantages and disadvantages of the complex, the existing problems and its significance in subsequent experiments and clinical studies Research methods: 1 Adipose stem cells were cultured in vitro and their biological characteristics were observed. 2 Astragalus polysaccharides collagen was prepared and the characteristics of collagen were detected. (3) Astragalus polysaccharide collagen was implanted into adipose stem cells. The biocompatibility of adipose stem cells with Astragalus polysaccharide collagen was studied by means of light microscope, adhesion test and cell proliferation. The content of VEGF secreted by Astragalus polysaccharides collagen complex was measured by 4ELISA method. 5 chicken embryo allantoic membrane test was used to detect the effect of adipose stem cells and Astragalus polysaccharides collagen complex on promoting angiogenesis. Results of the study: 1 Biological study of adipose stem cells: the cultured adipose stem cells have good morphology and good growth. 2 biocompatibility of Astragalus polysaccharide collagen with adipose stem cells: adipose stem cells can adhere, grow and proliferate on the scaffold of Astragalus polysaccharides collagen, and the adhesion rate of adipose stem cells is 10%, and adipose stem cells can grow and proliferate normally on the scaffold of Astragalus polysaccharide collagen. And Astragalus polysaccharides collagen can promote the proliferation of adipose stem cells at a certain stage. 3 Astragalus polysaccharides collagen and adipose stem cells co-cultured can promote the cytokine VEGF secreted by adipose stem cells. 4Adipose stem cells and Astragalus polysaccharides collagen complex could promote angiogenesis of chicken embryo allantoic membrane, and it was significantly higher than that of control group. The study concluded that: Astragalus polysaccharide collagen has good biocompatibility with adipose stem cells, and it can be an ideal scaffold carrier for adipose stem cells. The composite has the effect of promoting angiogenesis, and its mechanism may be related to cytokines secreted by adipose stem cells and lateral differentiation of adipose stem cells. There can be further research in this area.
【学位授予单位】：南京中医药大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R318.08

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