BMP-2及FGF-2对骨髓间充质干细胞成骨分化的影响

发布时间：2018-05-08 13:43

本文选题：骨形态发生蛋白- + 碱性成纤维细胞生长因子-　；参考：《重庆医科大学学报》2017年12期

【摘要】：目的:研究骨形态发生蛋白-2(bone morphogenetic protein-2,BMP-2)和碱性成纤维细胞生长因子-2(fibroblast growth factor-2,FGF-2)单独或联合应用对大鼠骨髓间充质干细胞(bone mesenchymal stem cells,BMSCs)增殖和成骨分化的诱导作用。方法:体外培养大鼠骨髓间充质干细胞,分别用不同浓度的BMP-2(10.0、100.0、200.0 ng/mL)和FGF-2(0.1、1.0、10.0 ng/mL)单独或联合诱导其增殖和成骨分化,于培养的第3、7天检测细胞增殖,第7天检测细胞周期分布,第7、14天检测碱性磷酸酶(alkaline phosphatase,ALP)表达量,第14、21天采用茜素红染色检测钙结节的形成情况。结果:单独应用BMP-2能够明显促进BMSCs成骨分化,最佳剂量为200.0 ng/mL。当BMP-2为200.0 ng/mL时,ALP mRNA表达量为2.29±0.14(P0.01),钙结节形成达2.68±0.45(P0.01)。单独应用FGF-2能够明显促进BMSCs增殖,最佳剂量为1.0 ng/mL(P0.01)。联合应用BMP-2和FGF-2时,较低浓度就能明显促进BMSCs的增殖和分化(BMP-2为10.0 ng/mL,FGF-2为0.1 ng/mL),ALP mRNA表达量为2.52±0.15(P0.01),钙结节形成达3.18±0.45(P0.01),具有显著的协同诱导效应。结论:BMP-2可促进BMSCs向成骨分化,FGF-2可促进BMSCs增殖,两者联合应用可协同诱导BMSCs增殖和成骨分化。
[Abstract]:Aim: to study the effects of bone morphogenetic protein-2bone morphogenetic protein-2BMP-2) and basic fibroblast growth factor-2FGF-2 on the proliferation and osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs). Methods: rat bone marrow mesenchymal stem cells were cultured in vitro. The proliferation and osteogenic differentiation of rat bone marrow mesenchymal stem cells were induced by different concentrations of BMP-2 (10.0100.00.200.0ng / mL) and FGF-2P (0.1 mg / mL) alone or jointly. Cell proliferation and cell cycle distribution were detected on the 37th day and the 7th day, respectively. The expression of alkaline phosphatase (ALP) was detected on day 7 and day 14, and the formation of calcium nodules was detected by alizarin red staining on day 14. Results: BMP-2 alone could significantly promote the osteogenic differentiation of BMSCs. The best dose was 200.0 ng / mL. When BMP-2 was 200.0 ng/mL, the expression of ALP mRNA was 2.29 卤0.14, P0.01a, and the formation of calcium nodules was 2.68 卤0.45p0.01. FGF-2 alone could significantly promote the proliferation of BMSCs, and the best dose was 1.0 ng / mL P0.01g. When combined with BMP-2 and FGF-2, the low concentration of BMP-2 could significantly promote the proliferation and differentiation of BMSCs. The expression of BMP-2 was 10.0 ng / mLGF-2 = 0.1 ng / mLGF-2, the expression of ALP mRNA was 2.52 卤0.15 P0.01G, and the formation of calcium nodules was 3.18 卤0.45P0.01. it had a significant synergistic induction effect. Conclusion FGF-2 can promote the proliferation of BMSCs to osteogenic differentiation and FGF-2 can promote the proliferation of BMSCs, and the combination of BMP and BMP-2 can induce BMSCs proliferation and osteogenic differentiation in combination.
【作者单位】：重庆医科大学药学院药剂学教研室;福建省生物化工技术重点实验室;
【基金】：国家自然科学基金资助项目(编号:81401535)
【分类号】：R318.08

【相似文献】