大鼠脂肪源性干细胞与三维打印明胶支架的相容性

发布时间：2018-05-11 08:04

本文选题：脂肪源性干细胞 + 三维打印明胶支架　；参考：《解剖学报》2017年02期

【摘要】：目的通过观察大鼠脂肪源性干细胞(ADSCs)与三维打印(3DP)及戊二醛交联的明胶支架的相容性,筛选出最适合细胞生长的支架孔径,为进一步构建组织工程化组织或器官提供实验依据。方法采用酶消化法分离提取大鼠ADSCs,并用流式细胞术和多向诱导分化的方法进行鉴定;然后与不同孔径的3DP明胶支架复合培养,并用扫描电子显微镜和透射电子显微镜观察其超微结构,细胞活力分析仪检测其存活率;用MTT法检测二维(2D)与三维(3D)培养对ADSCs细胞活力的影响。结果获得的ADSCs具有多向分化潜能,具备干细胞的基本特征。接种ADSCs到3DP明胶支架后,扫描电子显微镜可看到细胞呈椭圆形或纺锤形,区别于传统二维培养的梭形形态;透射电子显微镜可看到细胞在支架空隙内散在分布,细胞核、细胞器等结构清晰,表明其与支架的相容性良好;90μm孔径的支架细胞存活率最高;3D培养的方法更有利于维持ADSCs的活力。结论 ADSCs与3DP明胶支架的相容性良好,90μm孔径的支架最适合ADSCs的生长。
[Abstract]:Objective to investigate the compatibility of rat adipogenic stem cell (ADSCs) with three dimensional printing (3DP) and glutaraldehyde crosslinked gelatin scaffolds, and to select the most suitable scaffolds for cell growth, and to provide experimental evidence for further construction of tissue engineered tissues or organs. Methods Rat ADSCs were isolated by enzyme digestion, identified by flow cytometry and multidirectional differentiation, then co-cultured with 3DP gelatin scaffolds with different pore sizes. The ultrastructure was observed by scanning electron microscope (SEM) and transmission electron microscope (TEM), the viability of ADSCs cells was detected by cell viability analyzer, and the effects of 2-D and 3D) culture on the viability of ADSCs cells were detected by MTT assay. Results the obtained ADSCs has the potential of multiple differentiation and has the basic characteristics of stem cells. After inoculating ADSCs to 3DP gelatin scaffold, scanning electron microscope (SEM) showed that the cells were oval or spindle-shaped, which was different from the spindle shape of traditional two-dimensional culture. The structure of organelle was clear, which indicated that the best cell survival rate of the scaffold with 90 渭 m pore diameter was the highest. The method of 3D culture was more favorable to maintain the viability of ADSCs. Conclusion the scaffold with 90 渭 m pore diameter of ADSCs and 3DP gelatin is the most suitable for the growth of ADSCs.
【作者单位】：中国医科大学公共基础学院组织工程学教研室;锦州医科大学人体解剖学教研室;
【基金】：国家自然科学基金(31600793)
【分类号】：R318.08

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