骨诱导碳纤维复合材料人工颅骨板急性期生物相容性研究

发布时间：2018-05-12 21:42

  本文选题：碳纤维复合材料 + 生物相容性　； 参考：《山西医科大学》2017年硕士论文

【摘要】：目的:验证骨诱导碳纤维复合材料急性期的生物相容性。方法:1.细胞毒性实验:采用材料浸提液培养细胞,观察其生长情况,用CCK-8试剂盒测量细胞的吸光度值并计算细胞相对增殖率;2.急性毒性实验:通过腹腔注射浸提液,观察ICR小鼠活动情况;3.微核实验:通过腹腔注射浸提液,检测ICR小鼠骨髓中的微核率。4.染色体畸变实验:通过腹腔注射浸提液,并用秋水仙素处理后,检测ICR小鼠染色体畸变情况。5.皮内刺激实验:将浸提液注入新西兰大白兔背部皮下,观察皮肤反应;6.热原反应实验:将浸提液通过耳缘静脉注入新西兰大白兔体内,观察体温变化;7.溶血实验:将浸提液加入新西兰大白兔抗凝血中,观察溶血情况并测量吸光度值;8.致敏实验:将浸提液注入豚鼠背部皮下诱导,在腹侧激发致敏,观察皮肤情况及病理变化。结果:1.细胞毒性实验:细胞长梭形,生长状况良好,A、B、C组在不同时间段的相对增殖率均75%;2.急性毒性实验:A、B、C组的小鼠活动良好,无不良反应;3.微核实验:A、B、C组的微核率分别为1.40‰、1.80‰、1.20‰;4.染色体畸变实验:A、B、C组无染色体发生畸变;5.皮内刺激实验:A、B、C组的平均原发刺激指数均小于0.4;6.热原反应实验:A、B、C组的体温升高值均0.6℃,并且每组3只兔子体温升高值总数1.4℃;7.溶血实验:A、B、C组溶血率均小于标准的5%;8.致敏实验:A、B、C组的致敏率为0,并且病理切片未见异常。结论:骨诱导碳纤维复合材料人工颅骨板无细胞毒性,不引起急性毒性反应,无遗传毒性,对皮肤无刺激,不会引起机体发热,无致敏性,符合国家医用材料临床应用的标准。
[Abstract]:Objective: to verify the biocompatibility of bone-induced carbon fiber composites in acute stage. Method 1: 1. Cytotoxicity test: the cells were cultured in the material extract, the growth of cells was observed, the absorbance value of cells was measured by CCK-8 kit and the relative proliferation rate of cells was calculated. Acute toxicity test: the activity of ICR mice was observed by intraperitoneal injection of extractant. Micronucleus test: the micronucleus rate in bone marrow of ICR mice was detected by intraperitoneal injection of extract. 4. 4. Chromosome aberration test: the chromosome aberration of ICR mice was detected by intraperitoneal injection of extract and colchicine treatment. Intradermal stimulation: the extract was injected subcutaneously into the back of New Zealand white rabbits and the skin reaction was observed. Pyrogen reaction experiment: the extract was injected into New Zealand white rabbits through auricular vein to observe the change of body temperature. Hemolysis experiment: the extract was added to the anticoagulant of New Zealand white rabbit, the hemolysis was observed and the absorbance value was measured. Sensitizing experiment: the extract was injected into the back of guinea pig to induce subcutaneously, and the skin condition and pathological changes were observed. The result is 1: 1. Cytotoxicity test: the cells were long fusiform and the growth condition was good. The relative proliferation rate of group C was 75 ~ 2 / 2 at different time points. In the acute toxicity test, the mice in the group B: AZB C had good activity and had no adverse reactions (P < 0. 05). The micronucleus rate of micronucleus test in group C was 1.40 鈥，

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