角膜修复材料渗透性能的研究

发布时间：2018-05-13 10:04

本文选题：角膜修复材料 + 壳聚糖　；参考：《华南理工大学》2012年硕士论文

【摘要】：角膜维持其基本形态和功能，需要足够的氧气和营养物质。但角膜缺乏血管，其所需物质主要通过空气、泪液和房水提供，，而这些营养物质主要通过扩散作用进入角膜组织内部。营养物质能否通过角膜修复材料充足的运输、扩散到角膜内部，对材料的正常使用十分重要，因此，渗透性能是角膜修复材料的重要指标。本研究以渗透扩散原理为基础，设计安装角膜修复材料渗透性能测定装置，通过制备一系列角膜材料，利用自制装置测定其对氧气、葡萄糖、色氨酸、氯化钠的渗透性，通过数据比较分析，研究材料性质、混合结构、混合比例等因素对角膜材料的渗透性能的影响，探讨渗透性能的影响机理。以气相色谱仪作为核心的氧气量检测装置，设计并安装了符合角膜修复材料透氧性能测定要求的色谱法透氧系数检测装置。分别测定了低、中、高三种透氧性能的材料，通过与Illinois8003等压法透氧仪所测定的数据比较说明，在10-15到10-12mLO_2·cm·cm~(-2)·s~(-1)·Pa~(-1)的范围内具有适用性和一定的准确性。根据池膜渗透法原理设计了渗透池装置以测定溶液中小分子物质在膜中的渗透系数，数据说明该装置在测定葡萄糖、色氨酸、氯化钠三种物质在膜中的渗透系数时符合溶解——扩散原理和池膜渗透原理，具有一定的适用性和重复性。制备了四种脱乙酰度的壳聚糖膜，并测定了膜对氧气、葡萄糖、色氨酸、氯化钠的渗透性能。脱乙酰度从77.1%增加到96.7%，壳聚糖的透氧系数从4.01×10-15mLO_2·cm·cm~(-2)·s~(-1)·Pa~(-1)下降到3.74×10-15mLO_2·cm·cm~(-2)·s~(-1)·Pa~(-1)，这主要是因为，随着脱乙酰度的提高，分子链规整度提高导致膜的结晶度有所提高，膜的结构趋于紧密，使氧气的渗透性能降低。但是，随着脱乙酰度的提高，壳聚糖膜的湿态透氧系数从0.73×10-12mLO_2·cm·cm~(-2)·s~(-1)·Pa~(-1)提高到2.01×10-12mLO_2·cm·cm~(-2)·s~(-1)·Pa~(-1)，葡萄糖的透过率从3.09×10~(-7)cm~2·s~(-1)提高到3.47×10~(-7)cm~2·s~(-1)，色氨酸的透过率从3.79×10~(-7)cm~2·s~(-1)提高到4.35×10~(-7)cm~2·s~(-1)，氯化钠的透过率从8.33×10~(-7)cm~2·s~(-1)提高到9.12×10~(-7)cm~2·s~(-1)。这是因为，随着脱乙酰度的提高，壳聚糖膜的平衡含水量提高，从而造成其物质渗透性提高。以EDC作为交联剂，制备了壳聚糖-胶原复合膜，并测定了膜对氧气、葡萄糖、色氨酸、氯化钠的渗透性能。随着胶原含量从0提高到45%，复合膜的湿态透氧系数从2.01×10-12mLO_2·cm·cm~(-2)·s~(-1)·Pa~(-1)提高到5.41×10-12mLO_2·cm·cm~(-2)·s~(-1)·Pa~(-1)，葡萄糖的渗透系数从3.47×10~(-7)cm~2·s~(-1)提高到10.67×10~(-7)cm~2·s~(-1)，色氨酸从4.2×10~(-7)cm~2·s~(-1)提高到12.45×10~(-7)cm~2·s~(-1)，氯化钠从9.12×10~(-7)cm~2·s~(-1)提高到32.14×10~(-7)cm~2·s~(-1)。这主要是因为，随着胶原用量的提高，复合膜的平衡含水量从48%提高到61%，从而提高了物质在复合膜中的渗透率。随着交联剂EDC用量的提高，复合膜的渗透性能有所下降，这主要是因为，交联剂的加入，提高了复合膜的交联度，使复合膜结构的紧密程度提高，降低了复合膜的平衡含水量，从而导致渗透性能降低。数据比较分析说明复合膜的渗透性能主要受其平衡含水量的影响。
[Abstract]:The cornea maintains its basic form and function, requiring sufficient oxygen and nutrients. But the cornea is lacking in blood vessels. The material is supplied mainly through air, tears, and aqueous humor. These nutrients are mainly through the diffusion into the cornea. Can the nutrients be transported to the cornea through the corneal repair material enough to spread into the cornea It is important for the normal use of materials. Therefore, permeability is an important indicator of corneal repair materials. Based on the principle of permeation and diffusion, this study designed and installed a device for determining the permeability of corneal repair materials. By preparing a series of corneal materials, it was used to determine the permeability of oxygen, glucose, tryptophan and sodium chloride. Through comparison and analysis of data, the influence of material properties, mixed structure, mixing ratio and other factors on the permeability of corneal materials is studied, and the mechanism of permeability is discussed.
Using the gas chromatograph as the core of the oxygen measuring device, a chromatographic method for detecting oxygen permeability coefficient is designed and installed. The materials of low, medium and high oxygen permeable properties are measured, respectively, by comparison with the data measured by the Illinois8003 isobaric oxygen permeable instrument, from 10-15 to 10-12m The applicability of LO_2 cm cm~ (-2) s~ (-1) Pa~ (-1) is applicable and certain accuracy.
According to the principle of cell membrane permeability method, a permeation pool device is designed to determine the permeability coefficient of medium and small molecular substance in the membrane. The data shows that the device conforms to the solubility of the three substances in the membrane of glucose, tryptophan and sodium chloride in the membrane, which has a certain applicability and repeatability.
Four deacetylation of chitosan membranes were prepared, and the permeability of the membrane to oxygen, glucose, tryptophan and sodium chloride was measured. The degree of deacetylation increased from 77.1% to 96.7%. The oxygen permeability coefficient of chitosan decreased from 4.01 x 10-15mLO_2. Cm~ (-2). S~ (-1). Pa~ (-1) to 3.74 * 10-15mLO_2. Cm. Cm~ (-2). With the increase of the degree of deacetylation and the increase of the molecular chain regularity, the crystallinity of the membrane is improved, the structure of the membrane tends to tighten and the permeability of oxygen is reduced. However, with the increase of the degree of deacetylation, the wet oxygen permeability coefficient of the chitosan film is increased from 0.73 * 10-12mLO_2. Cm. Cm~ (-2) to s~ (-1) Pa~ (-1) to 2.01 * 10-12mLO_2. Cm. Cm~ (-2). -1) Pa~ (-1), the transmittance of glucose increased from 3.09 x 10~ (-7) cm~2 s~ (-1) to 3.47 * 10~ (-7) cm~2 s~ (-7). The transmittance of tryptophan increased from 3.79 x to 4.35. The equilibrium water content of chitosan membrane increased, resulting in increased permeability of the material.
The chitosan collagen composite membrane was prepared with EDC as a crosslinker, and the permeability of the membrane to oxygen, glucose, tryptophan and sodium chloride was measured. With the increase of collagen content from 0 to 45%, the wet oxygen permeability coefficient of the composite membrane was increased from 2.01 * 10-12mLO_2. Cm. Cm~ (-1). Pa~ (-1) to 5.41 * 10-12mLO_2. Cm cm~. The osmotic coefficient of glucose increased from 3.47 x 10~ (-7) cm~2 / s~ (-1) to 10.67 x 10~ (-7) cm~2. S~ (-1), and tryptophan increased from 4.2 x 10~ (-7) to 32.14. The water content increased from 48% to 61%, thus increasing the permeability of the material in the composite membrane. With the increase of the crosslinking agent EDC, the permeability of the composite film decreased. This is mainly because the crosslinking agent is added to improve the crosslinking degree of the composite membrane, the tightness of the composite membrane structure is increased, the balance water content of the composite film is reduced, and the balance water content of the composite film is reduced. Data analysis shows that the permeability of the composite membrane is mainly affected by its equilibrium moisture content.

【学位授予单位】：华南理工大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.08;TB39

【参考文献】