新型鼻腔填塞材料聚乙二醇双丙烯酸酯复合物水凝胶的细胞相容性

发布时间：2018-05-13 14:54

本文选题：生物相容性材料 + 聚乙烯二醇类　；参考：《中国组织工程研究》2017年10期

【摘要】：背景:前期研究发现聚乙二醇双丙烯酸酯和壳聚糖形成的复合物可生物降解吸收,具有抗炎消肿能促进创面愈合作用,且生物相容性好,然而该复合物质地较脆,鉴于鼻腔解剖结构,需要一种具有力学支撑性能的材料来克服这种缺陷。目的:观察新型可降解吸收鼻腔填塞材料聚乙二醇双丙烯酸酯复合物水凝胶的细胞相容性。方法:通过光交联将聚乙二醇双丙烯酸酯、壳聚糖、聚乙烯醇材料复合,形成聚乙二醇双丙烯酸酯复合物水凝胶,根据国际通行医疗器械生物学评价标准采用直接解触法及间接浸提法评价其细胞相容性。(1)直接解触法:将聚乙二醇双丙烯酸酯复合物水凝胶与小鼠成纤维细胞L929共培养24 h,观察形态变化;(2)间接浸提法:分别以100,50μL聚乙二醇双丙烯酸酯复合物水凝胶浸提液及100μL RPMI1640培养液培养小鼠成纤维细胞L929,CCK-8法检测培养1-3 d的增殖及细胞毒性。结果与结论:(1)直接解触法:材料周围细胞形态呈伴突起的梭形或星形的扁平状结构,贴壁性生长,细胞饱满且折光性强,整体呈生长趋势,可见该材料生物相容性良好;(2)间接浸提法:随着时间的增加,含复合物水凝胶浸提液组细胞数量增加,细胞相对增殖率逐渐增加,细胞毒性为0至1级;(3)结果表明:聚乙二醇双丙烯酸酯复合物水凝胶材料具有良好的细胞相容性。
[Abstract]:Background: previous studies have shown that the complex formed by polyethylene glycol bisacrylates and chitosan can be biodegradable and absorbable, has anti-inflammatory and swelling effects, and has good biocompatibility, but the texture of the complex is brittle. In view of the anatomical structure of the nasal cavity, a mechanical support material is needed to overcome this defect. Aim: to observe the cytocompatibility of a new biodegradable nasal packing material polyethylene glycol diacrylate complex hydrogel. Methods: Polyethylene glycol bisacrylates, chitosan and polyvinyl alcohol were mixed by photocrosslinking to form polyglycol bisacrylates hydrogels. According to the international standard of biological evaluation of medical devices, direct desatalysis method and indirect extraction method were used to evaluate their cytocompatibility.) direct desaturation method: Polyethylene glycol diacrylate complex hydrogel and mouse fibroblast L929 were treated with polyglycol diacrylate complex hydrogel. After 24 hours of co-culture, morphological changes were observed. Indirect extraction method: the proliferation and cytotoxicity of mouse fibroblasts cultured in 100 渭 L polyethylene glycol diacrylate complex hydrogel solution and 100 渭 L RPMI1640 medium were detected by L929CCK-8 method for 1-3 days. Results and conclusion: the cell around the material showed fusiform or starlike flat structure with protuberance, adherent growth, full cells and strong refraction. It can be seen that the material has good biocompatibility and indirect extraction method: with the increase of time, the number of cells and the relative proliferation rate of the cells increased with the increase of time. The results of cytotoxicity from 0 to 1) showed that the hydrogels of polyethylene glycol diacrylate complex had good cytocompatibility.
【作者单位】：首都医科大学附属北京同仁医院耳鼻咽喉头颈外科耳鼻咽喉头颈科学教育部重点实验室(首都医科大学);
【基金】：北京市医院管理局“使命”人才计划项目(SML20150201)~~
【分类号】：R318.08

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