流体剪应力作用下VWF-A1A2A3介导的血小板P-选择素的原位表达

发布时间：2018-05-13 21:45

本文选题：血小板P-选择素 + 血管性血友病因子　；参考：《医用生物力学》2017年02期

【摘要】：目的探讨流体剪应力(fluid shear stress,FSS)对VWF-A1A2A3介导的血小板表面P-选择素表达的影响。方法利用平行平板流动腔实验系统,以CD62P:FITC作为血小板表面P-选择素表达的指示剂,采用荧光显微镜观察分析在不同FSS条件(0、1、2 Pa)下,血小板经由VWF-A1A2A3特异介导黏附后P-选择素表达随时间的变化过程,提取特征参数。结果 FSS扣动了血小板激活并产生P-选择素表达的扳机,激活比率受FSS大小和持续时间的正向调节,在1 Pa和2 Pa时的最高激活比率分别为9.42%和14.59%;P-选择素表达水平随FSS作用时间的延长先提高后降低,呈现一个"双相"的变化过程,最佳作用时间为7.5 min;P-选择素表达的荧光峰值随剪应力的增加而上升。结论血小板P-选择素表达受FSS和VWF-A1A2A3的协同调控,表达水平与力信号的持续时间相关。
[Abstract]:Objective to investigate the effect of fluid shear stress (FSS) on the expression of P- selectin on platelet surface mediated by VWF-A1A2A3. Methods CD62P:FITC was used as the indicator of P- selectin expression on platelets with parallel plate flow chamber system. Fluorescence microscope was used to observe and analyze the expression of P- selectin under different FSS conditions. The changes of P-selectin expression over time after VWF-A1A2A3-specific adhesion were used to extract the characteristic parameters. Results FSS pulled the trigger of platelet activation and produced P- selectin expression. The activation rate was positively regulated by the size and duration of FSS. The highest activation rates at 1 Pa and 2 Pa were 9.42% and 14.59%, respectively. The expression of P- selectin increased first and then decreased with the prolongation of FSS, showing a "biphasic" process. The fluorescence peak of P- selectin expression increased with the increase of shear stress. Conclusion the expression of P-selectin in platelets is regulated by FSS and VWF-A1A2A3, and the expression level is related to the duration of force signal.
【作者单位】：华南理工大学生物科学与工程学院生物力学研究所;
【基金】：国家自然科学基金项目(31170887,11272125,11432006)
【分类号】：R318.01

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