脂肪干细胞在人工骨支架诱导下的成骨效应

发布时间：2018-05-15 05:08

本文选题：组织支架 + 皮下脂肪　；参考：《中国组织工程研究》2015年28期

【摘要】：背景:种子细胞的来源不足是限制组织重建及再生医学发展的重要因素。目的:探索脂肪干细胞与不同种类人工骨混合后的诱导成骨效果。方法:从接受美容手术的女性志愿者腹部皮下抽取液状脂肪,分离脂肪干细胞。取第4代人脂肪间充质干细胞分为单纯成骨诱导液组、成骨诱导液+羟基磷灰石组、成骨诱导液+可吸收骨材料组、成骨诱导液+重组异种骨组。后3组分别加入羟基磷灰石生物陶瓷、可吸收骨材料和重组异种骨,且每组依据干预物浓度的差异分为3,10,20 g/L 3个亚组,每3 d换液1次.共进行12 d的诱导。结果与结论:成骨诱导液+羟基磷灰石组的洗脱液钙浓度显著高于单纯成骨诱导液组(P0.05);低浓度条件下成骨诱导液+可吸收骨材料组洗脱液钙浓度明显高于单纯成骨诱导液组(P0.05),但高浓度成骨诱导液+可吸收骨材料组洗脱液钙浓度与单纯成骨诱导液组差异无显著性意义(P0.05);成骨诱导液+重组异种骨组洗脱液钙浓度显著低于单纯成骨诱导液组(P0.05)。表明不同类型人工骨支架可以影响脂肪干细胞诱导成骨效果,其中以羟基磷灰石为支架可以获得较好的诱导成骨效果。
[Abstract]:Background: insufficient source of seed cells is an important factor limiting the development of tissue reconstruction and regenerative medicine. Objective: to explore the osteogenic effect of adipose stem cells mixed with different artificial bone. Methods: the liquid fat was extracted from the abdomen of female volunteers undergoing cosmetic surgery and adipose stem cells were isolated. The fourth generation of human adipose mesenchymal stem cells were divided into four groups: osteogenic inducer group, osteoblast inducer hydroxyapatite group, osteoblastic inducer absorbable bone material group, osteogenic induction fluid recombination xenograft group. Hydroxyapatite bioceramics were added to the latter three groups, which could absorb bone material and recombine heterologous bone. Each group was divided into 3 groups according to the difference of the concentration of intervention substance, 3 subgroups were divided into 3 subgroups, and the solution was changed once every 3 days. The induction lasted for 12 days. Results and conclusion: the concentration of calcium in the eluate of osteogenic inducer hydroxyapatite group was significantly higher than that in the pure osteogenic inducer group (P0.05), and the concentration of calcium in the eluate of osteogenic fluid absorbable bone material group was significantly higher than that of pure osteogenic material group at low concentration. However, there was no significant difference in the concentration of calcium in the eluate between the high concentration osteogenic inducer group and that in the pure osteogenic induction solution group, and the calcium concentration in the osteogenic fluid recombination xenograft group was significantly lower than that in the single bone group. Pure osteogenic fluid group (P0.05). The results showed that different types of artificial bone scaffolds could affect the osteogenic effect of adipose stem cells, in which hydroxyapatite was used as scaffold to obtain better osteogenic effect.
【作者单位】：内江市第二人民医院骨科;泸州医学院附属医院骨科;
【基金】：泸州医学院自然科学基金(2013ZRQN071)~~
【分类号】：R318.17

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