载NGF海藻酸凝胶-聚乳酸复合导管修复兔面神经缺损实验研究

发布时间：2018-05-15 15:11

本文选题：神经导管 + 面神经缺损　；参考：《中国临床解剖学杂志》2014年02期

【摘要】：目的观察载神经生长因子(nerve growth factors,NGF)海藻酸凝胶-聚乳酸复合导管修复面神经缺损的作用。方法建立白兔面神经缺损模型,将面神经断端植入制备好的载NGF海藻酸凝胶-聚乳酸复合导管内2 mm,使面神经两断端之间距离为10 mm,将导管两端与神经固定缝合皮肤。再随机将12只大白兔分为术后4、8、12周组。实验结束后切开大白兔面神经吻合部位,选取神经导管近、中和远端组织进行HE染色,观察神经生长及导管降解情况。结果实验白兔手术伤口Ⅰ期愈合,无局部坏死、移植物排出及全身不良反应。术后4周导管壁有巨噬细胞浸润,导管近端雪旺细胞、成纤维细胞增生,新生小血管丰富;8周可见导管壁变薄,有成纤维细胞附着,巨噬细胞浸润,导管内有神经束样结构,束间纤维分隔,外层纤维包膜增厚,新生小血管减少;12周可见神经导管壁进一步分解,胶原纤维增生,导管内神经纤维结构接近正常,轴突样结构增粗,外层纤维包膜变薄。结论载NGF海藻酸凝胶-聚乳酸复合导管能较好的引导面神经再生,具有良好的组织相容性。
[Abstract]:Objective To observe the effect of nerve growth factors (NGF) alginate gel and polylactic acid compound catheter on the repair of facial nerve defect. Methods a rabbit facial nerve defect model was established, and the broken end of the facial nerve was implanted into the prepared NGF alginate gel - polylactic acid compound catheter, 2 mm, and the distance between the two ends of the facial nerve was 10 mm. At the end of the catheter, the skin was sutured with the nerve. 12 rabbits were randomly divided into 12 weeks after the operation. After the experiment, the facial nerve anastomosis site was cut through the rabbits. The nerve conduit was selected and the distal tissue was stained with HE, and the nerve growth and catheter degradation were observed. 4 weeks after the operation, the catheter wall was infiltrated by macrophages, the proximal end of the ductus Schwann cells, fibroblasts proliferated, the newborn small blood vessels were rich, and the catheter wall was thinner in the 8 week, the fibroblasts were attached, the macrophages were infiltrated, the intraductal fiber was separated, the outer fibers were thickened, and the outer fibrous capsule thickened. The decrease of the small vessels, the further decomposition of the nerve conduit wall, the proliferation of collagen fibers, the near normal nerve fiber structure in the catheter, the thickening of the axon like structure and the thinning of the outer fibrous capsule in 12 weeks. Conclusion the NGF alginate gel - polylactic acid compound catheter can guide the rebirth of the facial nerve better and have good histocompatibility.

【作者单位】：南方医科大学第三附属医院耳鼻咽喉头颈外科;
【基金】：广东省医学科研基金项目(A2010350)
【分类号】：R318.08

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