两种新型多孔骨组织工程支架的制备及性能研究

发布时间：2018-05-17 06:28

本文选题：骨组织工程 + 3D打印　；参考：《中国人民解放军医学院》2017年硕士论文

【摘要】：研究背景及目的:长期以来,节段性骨缺损的修复一直未得到有效地解决,成为目前骨科医生亟待解决的临床难题。骨组织工程学的迅速发展为临床骨缺损修复提供了新的思路。支架是骨组织工程最基本组成部分之一,如何构建出理想的支架一直是骨组织工程领域研究的热点。本研究基于不同材质,制备两种新型多孔骨组织工程支架:3D打印多孔钛支架(3D-STPT)及增强型BCP多孔陶瓷支架(RBCP);对两种新型多孔骨组织工程支架的形貌和微观结构、孔径和孔隙率、生物力学性能及生物组织相容性等方面进行检测;通过构建兔前臂桡骨临界性骨缺损模型对两种新型多孔骨组织工程支架的骨缺损修复能力进行初步评估。材料与方法:1、通过3D打印、表面化学处理、电化学沉积及双氧水发泡法分别制备出3D打印多孔钛支架(3D-STPT)及增强型BCP多孔陶瓷支架(RBCP),并对其形貌和微观结构、孔径和孔隙率、生物力学性能等进行评估。2、与MC3T3-E1细胞体外共同培养,对两种新型多孔骨组织工程支架进行生物组织相容性评估,初步评估两种新型多孔骨组织工程支架的生物组织相容性。3、通过构建兔桡骨临界性骨缺损模型对两种新型多孔骨组织工程支架的骨缺损修复能力进行初步评估。结果:1、SEM扫描结果显示:两种新型多孔骨组织工程支架均具备多孔结构,孔孔相连,孔径及孔隙率:3D打印多孔钛支架(3D-STPT)孔径范围为400μm-500μm,孔隙率为(66.0±0.7) %;增强型BCP多孔陶瓷支架(RBCP)孔径范围为100μm-500μm,孔隙率为(74.6±3.0) %,基本达到骨组织工程支架对孔径及孔隙率基本要求(孔径100 μm,孔隙率60%); X射线晶体衍射结果显示:两种新型多孔骨组织工程支架的表面成分与理论设计成分基本一致;生物力学性能测试结果显示:3D打印多孔钛支架(3D-STPT )压缩强度为83.14±0.96MPa,增强型BCP多孔陶瓷支架(RBCP)压缩强度为7.68±0.23MPa,两种新型多孔骨组织工程支架符合骨组织工程支架生物力学性能标准。2、CCK-8结果显示:两种新型多孔骨组织工程支架能够促进MC3T3-E1细胞体外增殖,具有较好生物组织相容性。3、兔桡骨临界性骨缺损修复实验显示:两种新型多孔骨组织工程支架在动物体内对临界性骨缺损具有较好骨缺损修复作用。结论:1、基于两种不同材质和3D打印技术,成功制备两种新型多孔骨组织工程支架:3D打印多孔钛支架(3D-STPT)和增强型BCP多孔陶瓷支架(RBCP),其孔径大小、孔隙率、生物力学性能基本符合骨组织工程支架的要求;2、两种新型多孔骨组织工程支架具有较好的生物组织相容性;3、两种新型多孔骨组织工程支架能够修复兔桡骨临界性骨缺损,具有潜在的临床应用价值。
[Abstract]:Background and objective: for a long time, the repair of segmental bone defects has not been effectively resolved, and has become a clinical problem to be solved urgently by orthopedic doctors. The rapid development of bone tissue engineering provides a new idea for clinical bone defect repair. Scaffold is one of the most basic components of bone tissue engineering. In this study, two new porous bone tissue engineering scaffolds, 3D-STPT3D-STPT3D-STPTand enhanced BCP porous ceramic scaffolds were prepared based on different materials, and the morphology and microstructure, pore size and porosity of the two new porous bone tissue engineering scaffolds were investigated. The biomechanical properties and biocompatibility of the two new porous bone tissue engineering scaffolds were evaluated by constructing a critical bone defect model of rabbit forearm radius. Materials and methods: 1. 3D printed porous titanium scaffolds 3D-STPTand enhanced BCP porous ceramic scaffolds were prepared by 3D printing, surface chemical treatment, electrochemical deposition and hydrogen peroxide foaming, respectively, and their morphology and microstructure, pore size and porosity were analyzed. The biomechanical properties were evaluated. 2. The biocompatibility of two new scaffolds of porous bone tissue engineering was evaluated by co-culture with MC3T3-E1 cells in vitro. The biocompatibility of two new porous bone tissue engineering scaffolds was preliminarily evaluated. The ability of repairing bone defects of two new types of porous bone tissue engineering scaffolds was preliminarily evaluated by constructing a rabbit radius critical bone defect model. Results the SEM scanning results showed that the two new types of porous bone tissue engineering scaffolds had porous structures, and the holes were connected to each other. The pore size and porosity of 3D printed porous titanium scaffold 3D-STPTis 400 渭 m-500 渭 m and 66.0 卤0.7 渭 m; the pore size range of enhanced BCP porous ceramic scaffold is 100 渭 m-500 渭 m and porosity is 74.6 卤3.0), which is basically up to the basic pore size and porosity of bone tissue engineering scaffold. The results of X-ray crystal diffraction show that the surface composition of the two new porous bone tissue engineering scaffolds is basically the same as that of the theoretical design. The results of biomechanical properties test showed that the compression strength of 3D-STPT was 83.14 卤0.96MPa, and the compressive strength of enhanced BCP porous ceramic scaffold was 7.68 卤0.23MPa. two new porous bone tissue engineering scaffolds accord with the biomechanical strength of bone tissue engineering scaffolds. The results of CCK-8 showed that two new scaffolds of porous bone tissue engineering could promote the proliferation of MC3T3-E1 cells in vitro. The experimental results of rabbit radius critical bone defect repair showed that two new porous bone tissue engineering scaffolds had good effect on repairing critical bone defects in animals. Conclusion: 1. Based on two different materials and 3D printing technology, two new porous bone tissue engineering scaffolds, 3D-STPTT) and enhanced BCP porous ceramic scaffolds, were successfully prepared. The pore size and porosity of the scaffolds were measured. The biomechanical properties basically meet the requirements of bone tissue engineering scaffolds. The two new porous bone tissue engineering scaffolds have good biocompatibility, and two new types of porous bone tissue engineering scaffolds can repair the critical bone defect of rabbit radius. It has potential clinical application value.
【学位授予单位】：中国人民解放军医学院
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R318.08;R68

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