紫外线对老化钛表面理化性能及生物活性的再激活作用

发布时间：2018-05-19 19:35

本文选题：牙种植体 + 钛　；参考：《中国组织工程研究》2017年22期

【摘要】：背景:随着储存时间的延长,钛种植体会出现老化现象,表面理化性能及生物活性会出现不同程度的下降,影响最终的种植体-骨结合效能,如何迅速有效的恢复钛表面的理化性能及生物活性值得深入研究。目的:探讨紫外线对老化钛表面理化性能及生物活性的再激活作用。方法:将经过喷砂酸蚀处理的钛片随机分为3组,A组为喷砂酸蚀处理后的新鲜钛片,B组为常温常压保存4周,C组为常温常压保存4周后紫外线照射15 min,采用表面轮廓测量仪、X射线光电子能谱分析仪、水接触角分析仪检测钛表面的粗糙度、元素构成及表面能。将MG63成骨细胞分别接种于3组钛片表面,接种30 min、1 h、2 h、4 h,以Hoechst33342荧光染色检测细胞黏附能力;接种1,3 d,采用MTS法检测细胞增殖。结果与结论:(1)3组钛表面的表面粗糙度无明显差异;(2)A、C组钛片表面C元素含量低于B组(P0.05),Ti、O、N元素含量显著高于B组(P0.05),A、C组钛片表面各种元素构成无明显差异;(3)A、C组钛片表面静态水接触角均为0°,B组钛片表面静态水接触角为115°;(4)接种30 min、1 h、2 h、4 h,A、C组钛片表面的细胞黏附数量高于B组(P0.05),A、C组钛片表面的细胞黏附数量无明显差异;(5)接种1,3 d,A、C组钛片表面的细胞增殖能力高于B组(P0.05),A、C组钛片表面的细胞增殖能力无明显差异;(6)结果表明,紫外线可激活老化钛表面的理化性能及生物活性,显著减少碳氢化合物的污染,恢复钛表面的高表面能,有利于细胞的黏附和增殖。
[Abstract]:Background: with the prolongation of storage time, titanium implantation experiences aging phenomenon, surface physical and chemical properties and biological activity will decrease in varying degrees, which will affect the ultimate implant-bone binding efficiency. How to restore the physical and chemical properties and biological activity of titanium surface quickly and effectively is worth further study. Objective: to study the reactivation effect of ultraviolet on the physical and chemical properties and biological activity of aging titanium surface. Methods: the titanium slices treated by sandblasting acid etching were randomly divided into three groups: group A: fresh titanium tablets treated by sand blast acid etching, group B: stored at room temperature and atmospheric pressure for 4 weeks, group C stored at room temperature and atmospheric pressure for 4 weeks, then irradiated with ultraviolet radiation for 15 min. Profile Meter X-ray photoelectron Spectrometer, The surface roughness, element composition and surface energy of titanium were measured by water contact angle analyzer. The MG63 osteoblasts were inoculated on the surface of three groups of titanium slices for 30 min, 1 hour, 2 h and 4 h, respectively. The adhesion ability of the cells was detected by Hoechst33342 fluorescence staining, and the proliferation of the cells was detected by MTS method at 1 day and 3 days after inoculation. Results and conclusion there is no significant difference in surface roughness between the two groups. The content of C element on the surface of group C is lower than that of group B (P 0.05) TiON is significantly higher than that of group B (P 0.05). There is no significant difference in the composition of elements on the surface of group A ~ (3). Static water contact angle of 0 掳B group was 115 掳/ L ~ (4) the number of cell adhesion on the surface of group C was higher than that of group B (P 0.05). There was no significant difference in the number of cell adhesion on the surface of titanium sheet in group A ~ (3) C) after inoculation for 30 min, 1 h, 2 h, 4 h, and 4 h after inoculation, and there was no significant difference in the number of cell adhesion on the surface of titanium sheet in group B (n = 5). The number of cell adhesion in group C was higher than that in group B (n = 5). The results showed that the proliferation ability of the cells on the surface of the film was higher than that on the surface of the titanium slice in group B (P 0.05). The results showed that the proliferation ability of the cells on the surface of the film was not significantly different from that of the group B. UV can activate the physical and chemical properties and biological activities of aging titanium surface, reduce the pollution of hydrocarbons, restore the high surface energy of titanium surface, and facilitate cell adhesion and proliferation.
【作者单位】：南方医科大学口腔医院(广东省口腔医院)牙周种植科;南方医科大学口腔医院(广东省口腔医院);
【基金】：国家自然科学基金(81600900) 广东省自然科学基金(2014A030310146)~~
【分类号】：R783.1

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