西藏猪模型对新型生物人工肝治疗安全性的评估

发布时间：2018-05-23 11:12

本文选题：肝 + 人工　；参考：《重庆医学》2014年09期

【摘要】：目的评估新型生物人工肝大动物实验的安全性。方法将6只西藏猪采用混合与单纯两种生物治疗模式,观察记录实验过程中每小时生命体征、静脉压、跨膜压、入浆壶压的变化,并在实验开始时及实验4、8h抽取静脉端血做内毒素及血培养检测。结果生物人工肝机器的静脉压、跨膜压、入浆壶压值在实验5h较实验开始时明显增高(P0.05),而其余各时间点与实验开始时比较差异无统计学意义(P0.05)。各时间点平均动脉压、呼吸频率比较差异无统计学意义(P0.05),实验2h心率与实验开始时比较差异有统计学意义(P0.01),实验开始时及实验4、8h抽血所测得的内毒素结果均小于0.5EU/mL,血培养结果显示培养15d未见细菌生长。结论新一代生物人工肝大动物实验安全,可以进一步评价猪的肝衰竭模型的有效性。
[Abstract]:Objective to evaluate the safety of new biological artificial liver in animal experiment. Methods six Tibetan pigs were treated with mixed and simple biotherapy models. The changes of vital signs, venous pressure, transmembrane pressure and pot pressure per hour were observed and recorded during the experiment. At the beginning of the experiment and 4 hours after the experiment, venous blood was drawn for endotoxin and blood culture. Results the venous pressure, transmembrane pressure and pot pressure of the biological artificial liver machine were significantly higher than that at the beginning of the experiment at 5 h, but there was no significant difference between the other time points and the beginning of the experiment. Mean arterial pressure at all time points, There was no significant difference in respiratory frequency (P 0.05). There was a significant difference in heart rate between the two groups at the beginning of the experiment and at the beginning of the experiment. The results of endotoxin taken at the beginning of the experiment and at the 8th hour of the experiment were all less than 0.5 EU. mL. the results of blood culture showed that the endotoxin was less than 0.5 EU. mL. No bacteria growth was observed at 15 days. Conclusion the new generation of biological artificial liver is safe and can be used to evaluate the effectiveness of the model of liver failure in pigs.
【作者单位】：南方医科大学珠江医院肝胆二科;
【基金】：国家高技术研究发展计划(863计划)资助项目(2006AA02A141,2012AA020505) 广东省中国科学院全面战略合作项目(2011B090300015) 广东省教育部产学研结合项目(2007B090400094) 广东省科技计划项目(2007A032100005)
【分类号】：R318.1

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