纳米银的抗菌特性及对多重耐药菌株的抗菌作用

发布时间：2018-05-25 17:34

本文选题：纳米银(silver + nanoparticles　；参考：《微生物学报》2017年04期

【摘要】：【目的】利用革兰氏阴性细菌、革兰氏阳性细菌和真菌的模式菌株分析纳米银的抗菌特性,并评价纳米银对多重耐药菌株的抗菌作用。【方法】利用生物法合成的纳米银,以微量肉汤法测定3种标准菌株的最小抑菌浓度(MIC)和最小杀菌浓度(MBC),并计算MBC/MIC比值。用系列浓度的纳米银处理3株标准菌株后经平板计数法绘制时间-杀菌曲线。采用菌落平板计数法测定了纳米银对3种标准菌株的"抗生素后效应"(post-antibiotic effect,PAE),最后在生物安全II级实验室测定纳米银对临床分离的多重耐药菌株的抗菌作用。【结果】用生物法合成了粒径5 30 nm的纳米银,zeta电位为 19.5 m V。该纳米银制剂对3种标准菌株的时间-杀菌曲线均表现为时间依赖型抗菌作用。纳米银对大肠杆菌和白色念珠菌"抗生素后效应"随着浓度增加而增加,对金黄色葡萄球菌无明显"抗生素后效应"。纳米银对3种标准菌株的MIC值和MBC值均在1.00 4.00μg/m L之间;对3株人源性多重耐药菌MIC值在6.00 26.00μg/m L之间,MBC值在1.00 32.00μg/m L之间;对14株动物源性多重耐药菌MIC值在4.00 10.00μg/m L之间,MBC值在8.00 16.00μg/m L之间。纳米银对所有测试菌株的MBC/MIC值均小于2。【结论】纳米银是一种时间依赖型的抗菌剂,有不同程度的"抗生素后效应",对人源和动物源性多重耐药菌有杀菌作用。
[Abstract]:[objective] to analyze the antibacterial properties of silver nanoparticles by Gram-negative bacteria, Gram-positive bacteria and fungal model strains, and to evaluate the antibacterial effect of silver nanoparticles against multidrug resistant strains. [methods] Nano-silver was synthesized by biological method. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) of three standard strains were determined by microbroth method, and the MBC/MIC ratio was calculated. The time-bactericidal curve was drawn by plate counting method after three standard strains were treated with a series of silver nanoparticles. Colony plate counting method was used to determine the "post-antibiotic effect" of silver nanoparticles on three standard strains, post-biotic effectPAE. Finally, the antimicrobial activity of silver nanoparticles against clinically isolated multidrug resistant strains was determined in biosafety II laboratory. The nanocrystalline silver zeta potential of 5 ~ 30 nm was prepared by biological method with a potential of 19.5 MV. The time-bactericidal curves of the nano-silver preparation against three standard strains were all time-dependent. The "post-antibiotic effect" of silver nanoparticles on Escherichia coli and Candida albicans increased with the increase of concentration, but had no obvious "post-antibiotic effect" on Staphylococcus aureus. The MIC and MBC values of silver nanoparticles to three standard strains were between 1.00 渭 g / mL and 4.00 渭 g / mL, and the MIC values of three human multidrug resistant strains were 6.00 ~ 26.00 渭 g / mL and 1.00 ~ 32.00 渭 g / mL, respectively. The MIC values of 14 strains of animal derived multidrug resistant strains were between 4.00 渭 g / mL and 10.00 渭 g / mL, and the values were between 8.00 渭 g / mL and 16.00 渭 g / mL. The MBC/MIC value of silver nanoparticles to all tested strains was less than 2. [conclusion] Silver nanoparticles is a time dependent antimicrobial agent with different degrees of "post-antibiotic effect", and has bactericidal effect on human and animal multidrug resistant bacteria.
【作者单位】：西南大学生物技术学院;中国疾病预防控制中心传染病预防控制所;
【基金】：中央高校基本业务费专项资金(XDJK2016E105)~~
【分类号】：R318.08

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