异种生物衍生骨材料与骨髓间充质干细胞的生物相容性

发布时间：2018-05-29 08:44

本文选题：生物相容性材料 + 干细胞　；参考：《中国组织工程研究》2015年28期

【摘要】：背景:异种生物衍生骨保存了原骨组织的天然网状孔隙结构,且具有免疫原性低、细胞相容性好的特点。目的:验证异种生物衍生骨材料与骨髓间充质干细胞的生物相容性。方法:取新鲜猪股骨制备生物衍生骨,扫描电镜观察材料结构。将第3代兔骨髓间充质干细胞以2×109 L-1的浓度接种于生物衍生骨材料的松质骨面,复合培养7 d内,采用扫描电镜观察细胞生长情况;复合培养8 d内,计数材料上附着的细胞数。结果与结论:生物衍生骨表面粗糙,孔隙不规则并相互通联,构成网状结构。复合培养3 d,细胞在衍生骨材料表面发生附着,且细胞形态不均一;复合培养培养5 d,细胞连接成片呈层状生长,细胞之间紧密接触;复合培养7 d,细胞呈现多层生长状态,成堆生长,部分区域存在细胞外基质分泌现象。复合培养后,前2 d为潜伏适应期,3-6 d细胞生长呈出线性曲线,处于细胞生长对数期;第6天后,细胞生长曲线逐渐变得平缓,细胞增殖速度下降,增殖进入平台期。表明异种生物衍生骨材料与骨髓间充质干细胞具有良好的生物相容性。
[Abstract]:Background: xenogeneic derived bone preserves the natural reticular pore structure of the original bone and has the characteristics of low immunogenicity and good cytocompatibility. Aim: to verify the biocompatibility of xenogeneic derived bone materials with bone marrow mesenchymal stem cells (BMSCs). Methods: the biological derived bone was prepared from fresh pig femur and the structure of the material was observed by scanning electron microscope (SEM). Rabbit bone marrow mesenchymal stem cells (BMSCs) of the third generation were inoculated at 2 脳 109 L-1 concentration on the cancellous bone surface of the biological derived bone material. The growth of the cells was observed by scanning electron microscope within 7 days and 8 days after co-culture. Count the number of cells attached to the material. Results & conclusion: the surface of biologically derived bone was rough, the pores were irregular and connected with each other, forming a reticular structure. After 3 days of co-culture, the cells were attached on the surface of the derived bone material, and the morphology of the cells was not uniform. After 5 days of co-culture, the connective cells grew in layers, and the cells were in close contact with each other. After 7 days of co-culture, the cells showed a multilayer growth state. In piles of growth, extracellular matrix secretion occurs in some regions. After co-culture, the cell growth curve showed a linear curve in the first 2 days of latent adaptation phase, which was in the logarithmic phase of cell growth, and after 6 days, the cell growth curve gradually became smooth, the cell proliferation rate decreased, and the cell proliferation entered the plateau phase. The results showed that the xenogeneic bone derived materials had good biocompatibility with bone marrow mesenchymal stem cells.
【作者单位】：上海交通大学附属上海市第一人民医院骨科;
【基金】：上海市科委重点项目(12441902402)~~
【分类号】：R318.08

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