明胶-壳聚糖-羟基磷灰石-米诺环素仿生纳米复合材料修复桡骨缺损

发布时间：2018-05-30 20:32

本文选题：壳聚糖 + 明胶　；参考：《中国组织工程研究》2015年30期

【摘要】：背景:课题组前期研制了新型明胶-壳聚糖-羟基磷灰石-米诺环素仿生纳米复合材料。目的:观察明胶-壳聚糖-羟基磷灰石-米诺环素仿生纳米复合材料修复兔桡骨缺损的效果。方法:取30只成年健康新西兰大白兔,在一侧桡骨上端制作约15 mm×6 mm的临界性腔隙性骨缺损,随机分为实验组(n=15)、自体骨移植组(n=10)和空白对照组(n=5),实验组将明胶-壳聚糖-羟基磷灰石-米诺环素仿生纳米复合材料植入骨缺损处,自体骨移植组从对侧桡骨取等长桡骨植入骨缺损处,空白对照组不做任何处理。于术后2,4,8,12周分别行大体观察、组织学观察及X射线平片检查。结果与结论:植入后12周,实验组骨缺损区有明显血管生成,骨性愈合完全,植入复合材料消失,出现板层骨结构,可见小血管,新生骨边缘与原骨边缘连接,表现成骨的连续性,密度较原骨稍降低,骨缺损区不明显;自体骨移植组骨性愈合,骨小梁重建较明显,板层骨较成熟,髓腔再通,骨折线完全消失,骨密度与原骨完全一致;空白对照组髓腔无明显成骨现象,大量纤维组织及炎症细胞浸润,骨折断端未愈合。表明明胶-壳聚糖-羟基磷灰石-米诺环素仿生纳米复合材料可明显促进临界性骨缺损修复,效果与自体骨移植相当。
[Abstract]:Background: a novel gelatin-chitosan-hydroxyapatite-minocycline biomimetic nanocomposite was developed. Aim: to observe the effect of gelatin-chitosan-hydroxyapatite-minocycline biomimetic nanocomposite in repairing rabbit radius defect. Methods: a critical lacunar bone defect of 15 mm 脳 6 mm was made in 30 healthy adult New Zealand rabbits at the upper end of the radius. The experimental group was randomly divided into two groups: the experimental group (n = 15), the autogenous bone graft group (n = 10) and the blank control group (n = 5). The experimental group implanted bone defect with gelatin, chitosan, hydroxyapatite and minocycline biomimetic nanocomposites. In the autograft group, the isometric radius was taken from the contralateral radius and implanted into the defect, while the blank control group was not treated. Gross observation, histological observation and X-ray plain-film examination were performed 12 weeks after operation. Results and conclusion: at 12 weeks after implantation, there was obvious angiogenesis in the bone defect area in the experimental group, the bone union was complete, the composite material disappeared, the lamellar bone structure appeared, small vessels could be seen, and the new bone margin was connected with the original bone edge. In autogenous bone transplantation group, bone healing, trabecular bone reconstruction, lamellar bone maturation, medullary cavity recanalization, fracture line disappeared completely, bone density was completely consistent with original bone. In the blank control group, there was no obvious osteogenesis, a large number of fibrous tissue and inflammatory cells infiltrated, and the broken end of fracture was not healed. The results showed that the biomimetic nanocomposites of gelatin chitosan hydroxyapatite and minocycline could promote the repair of critical bone defect and the effect was similar to that of autogenous bone graft.
【作者单位】：安徽医科大学附属第一医院骨科;安徽医科大学附属口腔医院;安徽口腔疾病研究省级实验室;
【分类号】：R318.08

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