釉基质蛋白对双重酸蚀后纯钛表面磷灰石生成的影响

发布时间：2018-05-31 06:08

本文选题：钛 + 牙釉质　；参考：《中国组织工程研究》2017年02期

【摘要】：背景:为提高钛种植体植入后的生物活性,常采用各种表面改性技术。目的:研究釉基质蛋白对双重热酸蚀纯钛表面仿生矿化沉积磷灰石层的影响。方法:提取猪未萌恒牙胚表面的釉基质蛋白并进行验证,纯钛片经抛光清洗后进行双重热酸蚀处理,然后放入含不同浓度釉基质蛋白的饱和矿化液中矿化7 d,对照组为不含釉基质蛋白的钙磷过饱和液。扫描电镜观察试件表面形貌,X射线能谱及X射线衍射分析涂层的元素成分及晶相结构。结果与结论:经双重酸蚀处理后的钛片表面形成规则凹坑状的粗糙表面,经过7 d的矿化实验后,不添加釉基质蛋白的对照组试件上未见明显矿化涂层的形成。加入釉基质蛋白的实验组试件表面可形成矿化涂层,且随釉基质蛋白浓度的增加,矿化涂层的量及形态发生改变。能谱分析结果显示:矿化涂层中的主要元素为钙、磷、碳和氧,钙磷摩尔比在1.32-1.41之间。X射线衍射结果显示纯钛试件表面的沉积物为碳羟基磷灰石。提示釉基质蛋白可以促进双重酸蚀处理后的纯钛表面更快地生成磷灰石涂层。釉基质蛋白对纯钛表面磷灰石涂层的影响呈浓度相关性。
[Abstract]:Background: in order to improve the bioactivity of titanium implants, various surface modification techniques are often used. Aim: to study the effect of enamel matrix protein on biomimetic mineralized apatite layer on the surface of pure titanium with double thermal acid etching. Methods: the enamel matrix protein was extracted from the surface of porcine immolative tooth germ and verified. The pure titanium plate was treated with double thermal acid etching after polishing and cleaning. Then it was mineralized in the saturated mineralized solution containing different concentration of enamel matrix protein for 7 days, and the control group was calcium phosphorus supersaturation solution without enamel matrix protein. Scanning electron microscopy (SEM) was used to observe the surface morphology and X-ray energy spectrum (EDS) and X-ray diffraction (XRD) of the coating to analyze the elemental composition and crystal phase structure of the coating. Results and conclusion: after double acid etching, the surface of titanium sheet formed regular crater rough surface. After 7 days of mineralization experiment, no obvious mineralized coating was formed on the control sample without adding enamel matrix protein. The mineralized coating could be formed on the surface of the experimental sample with the addition of enamel matrix protein, and the amount and morphology of the mineralized coating changed with the increase of the concentration of enamel matrix protein. The results of energy spectrum analysis show that the main elements in the mineralized coating are calcium, phosphorus, carbon and oxygen, and the molar ratio of calcium to phosphorus is between 1.32-1.41. The X-ray diffraction results show that the sediment on the surface of pure titanium sample is carbon-hydroxyapatite. The results suggest that the enamel matrix protein can accelerate the formation of apatite coating on the surface of pure titanium after double acid etching. The effect of enamel matrix protein on the apatite coating on pure titanium was concentration-dependent.
【作者单位】：武汉科技大学附属天佑医院口腔科;上海市东方医院口腔科;上海交通大学医学院附属第九人民医院口腔修复科;
【分类号】：R318

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