亲骨荧光素间接标记软骨内成骨的早期血管新生

发布时间：2018-06-07 08:59

本文选题：四环素 + 荧光　；参考：《中国组织工程研究》2017年08期

【摘要】：背景:虽然当前有多种方法可以观察和检测软骨内成骨早期血管新生,但是均存在一定的不足。目的:探讨四环素和茜素络合酮间接标记软骨内成骨过程中新生血管的可能性。方法:制备新西兰兔双侧桡骨骨缺损模型并植入β-磷酸三钙材料,分别于术后第1天和第15天注射四环素和茜素络合酮,第28天取材。部分标本在墨汁灌注后行硬组织切片荧光/光学显微镜观察,部分标本经脱钙后行免疫组化染色观察,比较软骨内成骨过程中亲骨荧光素标记管腔结构与免疫组化、墨汁灌注标记血管结构的一致性。结果与结论:(1)亲骨荧光素标记的管腔结构经免疫组化染色证实为CD34阳性的血管结构;(2)在荧光显微镜下,亲骨荧光素标记的血管形态与墨汁灌注的血管形态一致;荧光素标记后经墨汁灌注的血管在荧光显微镜下可见荧光管腔中有黑色墨汁走行;(3)此外,亲骨荧光素标记的管腔结构颜色绚丽、三维结构更加生动,可通过不同颜色的荧光显示不同时期的血管新生和演变过程,具有独特的优势,可用于软骨内成骨早期血管发生的形态学检测。
[Abstract]:Background: although there are many methods to observe and detect early angiogenesis in endochondral osteogenesis, there are some deficiencies. Aim: to investigate the possibility of indirect labeling of neovascularization with tetracycline and alizarin complex ketone during endochondral osteogenesis. Methods: the bilateral radial bone defect model of New Zealand rabbits was established and 尾 -tricalcium phosphate was implanted. Tetracycline and alizarin complex ketone were injected on the 1st and 15th day after operation, and the materials were obtained on the 28th day. Some of the specimens were observed by fluorescence / optical microscopy after infusing with Chinese ink, and others were observed by immunohistochemical staining after decalcification to compare the structure and immunohistochemistry of osteophile fluorescein labeling during endochondral osteogenesis. Consistency of blood vessel structure with ink perfusion labeling. Results and ConclusionThe vascular structure labeled with osteofluorescein was proved to be CD34 positive by immunohistochemical staining. Under fluorescence microscope, the morphology of the vessel labeled with osteofluorescein was consistent with that of the blood vessel infused with ink. Fluorescein labeled vessels infused with ink showed black ink in the fluorescent cavity under fluorescence microscope. In addition, the structure of the lumen labeled with osteofluorescein was colorful, and the three-dimensional structure was more vivid. It can display the process of angiogenesis and evolution at different stages by different color fluorescence, which has unique advantages and can be used to detect the morphology of angiogenesis in early stage of endochondral osteogenesis.
【作者单位】：广州医科大学附属武警广东省总队医院;
【基金】：国家自然科学基金(81303107) 广东省自然科学基金(S2012040006295) 广东省医学科研基金(A2012499;A2014499)~~
【分类号】：R318.08;R68

【相似文献】