生物骨组织立体培养分化和动物体内修复实验

发布时间：2018-06-07 20:01

本文选题：生物相容性材料 + 骨和骨组织　；参考：《中国组织工程研究》2017年06期

【摘要】：背景:体外培养组织工程骨仍是治疗骨缺损修复的重要途径,3D打印骨架材料结合生物反应器骨组织是骨组织工程的重要研究内容。目的:观察3D打印制备的生物骨支架接种细胞后经生物反应器立体培养体内修复骨伤的效果。方法:1配制生物骨材料聚乳酸-羟基乙酸共聚物/羟基磷灰石(PLGA/HA),3D打印和冷冻干燥技术制备骨架,接种人骨髓间充质干细胞、经生物反应器培养分化;2实验动物分为3组,分别为对照组(1号,2号),实验组(3号,4号),实验+空孔组(5号,6号,均行右股骨钻孔不植入任何材料);每组又分为2个亚组并编号。兔左侧股骨远端制作骨缺损模型,对照组分别植入空白材料-PLGA/HA(无细胞)和阴性材料PLGA/HA(接种人骨髓间充质干细胞)无诱导分化的支架块;实验组材料-PLGA/HA为接种人骨髓间充质干细胞经诱导分化的支架块,批号分别为201405~(-1)和201405~(-2);3电子显微镜确定生物骨材料的成骨和降解性能。CT扫描生物骨体内修改效果,待受试组的骨破损基本修复后,做组织病理学检查。结果与结论:制备出适于细胞种植的具有拓扑结构的生物骨支架;电子显微镜观察成骨干细胞附着的生物骨材料立体化培养后形成的新骨节点明显;在支架植入家兔体内后,有明显促进成骨愈合的作用。结果显示制备的生物骨组织材料达到了初步的修复骨组织的作用。
[Abstract]:Background: tissue engineering bone culture in vitro is still an important approach to repair bone defect. 3D printed skeleton material combined with bioreactor bone tissue is an important research content of bone tissue engineering. Aim: to observe the effect of 3 D printing scaffold on repairing bone injury after seeding cells in vivo with bioreactor. Methods the bone biomaterials, poly (lactic acid-glycolic acid) / hydroxyapatite / hydroxyapatite, were prepared by 3 D printing and freeze-drying techniques. Bone marrow mesenchymal stem cells were inoculated into human bone marrow mesenchymal stem cells. The experimental animals were divided into 3 groups by bioreactor culture. Control group (No.1, No.2), experimental group (No. 3, No. 4), experimental empty hole group (No. 5, No. 6), each group were divided into 2 subgroups and numbered. The model of bone defect was made at the distal end of the left femur in rabbits. In the control group, blank material PLGA / PA (without cells) and negative material PLGA / PA (inoculated with human bone marrow mesenchymal stem cells) were implanted into scaffolds without differentiation. The experimental group material -PLGA / HA was the scaffold of induced differentiation of human bone marrow mesenchymal stem cells (batch number 201405) and 201405TX / 2DX 3 electron microscope to determine the osteogenic and biodegradability of biomaterials. Ct scanning in vivo modification effect of biological bone. Histopathological examination was performed after the bone damage was repaired in the test group. Results and conclusion: a new bone scaffold with topologic structure suitable for cell implantation was prepared, and the new bone nodes formed after three-dimensional culture of bone materials attached to osteoblasts were observed by electron microscope. It can obviously promote the healing of osteogenesis. The results showed that the prepared biomaterials had the effect of repairing bone tissue.
【作者单位】：深圳职业技术学院应用化学与生物技术学院;吉林大学第二临床医学院;深圳大学医学院;
【基金】：广东省教育部项目(2012B091100408) 深圳市科技创新委项目(ZYA201106100083A;GGJS20130331152344401) 校级重点项目(601522k27009)~~
【分类号】：R318.08;R683

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