锌转运蛋白1 siRNA转染骨髓间充质干细胞对肿瘤坏死因子α信号成脂通路的影响

发布时间：2018-06-08 00:12

  本文选题：干细胞 + 股骨头坏死　； 参考：《中国组织工程研究》2017年13期

【摘要】：背景:课题组前期研究已经证实乙醇能促进骨髓间充质干细胞成脂分化,上调肿瘤坏死因子α成脂通路蛋白PPARγ,aP 2的表达。锌转运蛋白1(Zrt/Irt-like Protein 1,ZIP1)作为ZIP蛋白家族的成员与骨代谢和成骨分化有密切的关系。目的:观察ZIP1 siR NA转染的骨髓间充质干细胞对肿瘤坏死因子α信号成脂通路的影响。方法:分离培养兔骨髓间充质干细胞,使用0.03,0.09,0.15,0.21 mol/L乙醇进行培养,再使用ZIP1 siR NA转染骨髓间充质干细胞,同时对兔骨髓间充质干细胞进行ZIP1转染培养。结果与结论:(1)aP 2,PPARγ蛋白的表达:不同浓度乙醇培养aP 2,PPARγ蛋白表达均较明显升高(P0.05);除0.03 mol/L乙醇组之外,其他浓度乙醇组三酰甘油水平明显升高(P0.05);沉默ZIP1基因转染乙醇培养的骨髓间充质干细胞后,各乙醇浓度组a P2,PPARγ蛋白的表达量及三酰甘油水平均明显升高(P0.05),而骨髓间充质干细胞经ZIP1表达载体转染后,a P2,PPARγ蛋白表达明显降低(P0.05);(2)结果表明:沉默ZIP1表达能激活骨髓间充质干细胞的肿瘤坏死因子α信号成脂通路的表达,从而发挥促进细胞的成脂分化能力。
[Abstract]:Background: Previous studies have confirmed that ethanol can promote lipid differentiation in bone marrow mesenchymal stem cells and up regulate the expression of tumor necrosis factor alpha lipoprotein pathway protein PPAR gamma, aP 2. Zinc transporter 1 (Zrt/Irt-like Protein 1, ZIP1) as a member of the ZIP protein family has a close relationship with bone Xie Hecheng bone differentiation. Objective: To observe ZIP1 siR The effect of NA transfected bone marrow mesenchymal stem cells on the lipid pathway of tumor necrosis factor alpha. Methods: the rabbit bone marrow mesenchymal stem cells were isolated and cultured, 0.03,0.09,0.15,0.21 mol/L ethanol was used to culture, and ZIP1 siR NA was used to transfect bone marrow mesenchymal stem cells, and the rabbit bone marrow mesenchymal stem cells were transfected with ZIP1 transfection and culture. Results and conclusions: (1) expression of aP 2, PPAR gamma protein: the expression of aP 2 and PPAR gamma in ethanol culture at different concentrations increased significantly (P0.05), and the level of three acyl glycerol in other ethanol groups except 0.03 mol/L ethanol group was significantly increased (P0.05), and the concentration of ethanol concentration a P2, PPAR gamma after transfection of ZIP1 gene transfected with ethyl alcohol in bone marrow mesenchymal stem cells. The expression of protein and the level of three glycerol increased significantly (P0.05), while the expression of a P2 and PPAR gamma in bone marrow mesenchymal stem cells was significantly reduced after transfection of ZIP1 expression vector (P0.05). (2) the results showed that silent ZIP1 expression could activate the expression of swell necrosis factor alpha signaling pathway in bone marrow mesenchymal stem cells, and thus play an important role in promoting the expression of lipid pathway. The adipocyte differentiation capacity of the cell.
【作者单位】： 广西中医药大学附属瑞康医院骨科;广西中医药大学;
【基金】：广西自然科学基金课题(2015GXNSFAA139136) 广西高校青年教师基础能力提升项目(KY2016YB204) 广西卫生厅重点课题(S201419-05) 广西中医药大学校级科研项目(2015LX027) 2016年研究生教育创新计划资助项目立项课题(YJS201650) 2014年广西中医药大学校级培育学科-骨外科学建设项目[J13167(7)]~~
【分类号】：R318.08;R681.8
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本文编号：1993456